Sequences from confirmation amplicon


Confirmation sequences for line 432E01 and hit At3g48410 are shown.
Line ID 432E01
Locus At3g48410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17925838
Primers used for PCR 40yf - 3144, show primer details
   
Sequence Name 30-K119947-0022-432-E01C-40yf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 40yf: 5'-CTGATGGGTTAGGACTAGGACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K119947-0022-432-E01C-40yf
ATGGGCATGCCTTAACTACATTCCTCACAGGTACTGCATTTGAATCTGTCTTATTGGTTT
TGGAAATGTTTTCGATGTATCGAGAAAGCTATTAACTTTGATCTTGAAACAATAGCTAAG
ATGTGTTGTGAAATGTGTTTTTTTGGTTATGGACTTTGTTTACAGGTTAGCAGGAGCTGC
CCTTGTAGCTCCAGCGATTAACTATTGGTGGAGAAACTTACCGGGAGATTTAACAAGAGA
AGCTTTCTCTCTTATGCATCCTGCAGATCAATGGTCACTTCGAGTAGCTCATTATGCTCC
TTGGCTTACATATTGGTGGAACACTCAGAAATGGTTCCCAATCTCCAATGTGATTGCCGG
TAATCCCATTATTTTCTCACGTCAGGACATGGAGATCTTGTCGAAGCTCGGATTCGTCAA
TCCAAATCGGGTACAAGCACATCTATAGACGTTCAGTTTCCATATCATAAGTTTCATACC
TCTACAATGAGAAGATGATTTGGAATCATATGATGTGTATATTCCTTTTTTTTTGTTCCA
GGCATACATAAGACAACAAGGTGAATATGTAAGCTTACACCGAGATTTGAATGTCGCATT
TTCAAGCTGGGAGTTTGATCCGTTAGACCTTCAAGATCCGTTCCCGAACAACAATGGCTC
AGTTCACGTATGGAATGGCGATGAGGATAAGTTTGTGCCAGTAAAGCTTCAACGGTATGT
CGCGTCAAAGCTGCCATGGATTCGTTACCATGAAATATCTGGATCAGGACATTTTGTACC
ATTTGTGGAAGGTATGACTGATAAGATCATCAAGTC
   
Sequence Name 10-K119946-0022-432-E01C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119946-0022-432-E01C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGATA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGAA
TAATTGCAATGTTGTTAATTGTAATCTATGCGTTGATGTCTACATTTGCTCCCGTAAATG
CCGCCAGCCCTTTCATTTGAGACATTAGTACACTTGTATTAACGACTAAAGTTGCTTTGA
TTTGATTGTTGATCATAACTAGTTTAGCATTGCAAGCAATTAATTATTCTTAAAAACCAC
ATTGACTTAAACAGAGGCTTCTCTACTCTCACTTACATCTTCTTCCCCAACCAAAAGTGA
CTTGATGATCTTATCAGTCATACCTTCCACAAATGGTACAAAATGTCCTGATCCAGATAT
TTCATGGTAACGAATCCATGGCAGCTTTGACGCGACATACCGTTGAAGCTTTACTGGCAC
AAACTTATCCTCATCGCCATTCCATACGTGAACTGAGCCATTGTTGTTCGGGAACGGATC
TTGAAGGTCTAACGGATCAAACTCCCAGCTTGAAAATGCGACATTCAAATCTCGGTGTAA
GCTTACATATTCACCTTGTTGTCTTATGTATGCCTGGAACAAAAAAAAAGGAATATACAC
ATCATATGATTCCAAATCATCTTCTCATTGTAGAGGTATGAAACTTATGATATGGAAACT
GAACGTCTATAAATGTGCTTGTACCCGATTTGGATTGAC

Last Updated on 06.12.2018 16:11