Sequences from confirmation amplicon


Confirmation sequences for line 807G04 and hit At3g50500 are shown.
Line ID 807G04
Locus At3g50500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18742898
Primers used for PCR DB29 - 8474, show primer details
   
Sequence Name 55-K029917-022-807-G04C-8409
GenBank Accession LN508702 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K029917-022-807-G04C-8409
CCATTTCATTGAATATATTAACCAAAATCACATATTTTCAAACGAGGCGCTGGACTTCCA
TCTAACAATGTATTTTCCAGCTTCAGATCCCGATGGCATATTTGCTTCACAGGAAAATCA
AACACAGAATTAAAACCAGAGTGGTATACACGAGAACGAGAAAAGTTCAAGTAAGTTGCA
AGAACACTGCTCTGCGTACCATTGCATGACAATAGCTAACTCCAGATATAAGCTGCTGAA
AGAAGAACCGAGCCTATACATAAAAAAACTTAAAGATCAGTAGTCACACATCAATACCAC
AACACAGTACGATAAATCAGAATCCAAACCTCATCTTCACTAAACCGTCCGGCATTACAA
ATCCGCTCATAAAGTTCTCCACCAGCAGCATATTCCATAACAATAGCCAAATGGGAAGGC
GTCAAAATCACCTAAACCATTACAAAAACACACACAAAACAAACTCTAAACCACACTGAC
TTAATACACAAACACACACAGAA
   
Sequence Name 55-K029917-022-807-G04C-DB29
GenBank Accession LN508703 [GenBank]
Sequencing primer gene specific primer, DB29: 5'-ACACCTTGATGTTTCTTCTGTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K029917-022-807-G04C-DB29
TGTGTGTGTTTTTGTAATGGTTTAGGTGATTTTGACGCCTTCCCATTTGGCTATTGTTAT
GGAATATGCTGCTGGTGGAGAACTTTATGAGCGGATTTGTAATGCCGGACGGTTTAGTGA
AGATGAGGTTTGGATTCTGATTTATCGTACTGTGTTGTGGTATTGATGTGTGACTACTGA
TCTTTAAGTTTTTTTATGTATAGGCTCGGTTCTTCTTTCAGCAGCTTATATCTGGAGTTA
GCTATTGTCATGCAATGGTACGCAGAGCAGTGTTCTTGCAACTTACTTGAACTTTTCTCG
TTCTCGTGTATACCACTCTGGTTTTAATTCTGTGTTTGATTTTCCTGTGAAGCAAATATG
CCATCGGGATCTGAAGCTGGAAAATACATTGTTAGATGGAAGTCCGGCACCTCGTTTGAA
AATATGTGATTTTGGTTAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTAC
Primers used for PCR FN54 - 8474, show primer details
   
Sequence Name 50-K118414-0022-807-G04C-FN54
GenBank Accession LN507317 [GenBank]
Sequencing primer gene specific primer, FN54: 5'-AATGATGATGAATGAGACAGCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118414-0022-807-G04C-FN54
ATTGTCTTTCGATAATCTCGTGGCTCCTGTGGATCCTCGAATGGATAAGCTCCAACCAAC
ATTACATATAATGTTACACCGCAAGACCATACATCTGCAAGCTGGAAAAATGTAACACCA
GCTATTTCAGAGGACAACTCAAAGAATATTTGTGGTATAAACAGTCATATGGATAAGCTG
GTTTGAACTCTAGACAGAGGAATTGGTCTCATGGCCTTACCTTGCCATCATATTCCTGTC
GAAGAAGAATCTCTGGTGCAATGTATGCAGGAGTACCAACAGTTGACTTTGGTTGGGAAT
GAAGAACAGAAGACTGAAGAAAAGAAACGCATAGTTAATACTTGAGCAGAAATCAAAGCG
AAAACAGACACTTAGTAGGAAACACATGTAGGTAAAGAGCATGAACGATAAGAAGCACAA
CGAGGAATCACAAGATGTCCCCCGCTGTTCTGCGCTTGGCCATACAGTAGCATATATAAA
CTTAGAATTCAGAAAACAAACCTTGAGAGAAATAAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K118413-0022-807-G04C-8409
GenBank Accession LN487235 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118413-0022-807-G04C-8409
ATTTATTTCTCTCAAGGTTTGTTTTCTGAATTCTAAGTTTATATATGCTACTGTATGGCC
AAGCGCAGAACAGCGGGGGACATCTTGTGATTCCTCGTTGTGCTTCTTATCGTTCATGCT
CTTTACCTACATGTGTTTCCTACTAAGTGTCTGTTTTCGCTTTGATTTCTGCTCAAGTAT
TAACTATGCGTTTCTTTTCTTCAGTCTTCTGTTCTTCATTCCCAACCAAAGTCAACTGTT
GGTACTCCTGCATACATTGCACCAGAGATTCTTCTTCGACAGGAATATGATGGCAAGGTA
AGGCCATGAGACCAATTCCTCTGTCTAGAGTTCAAACCAGCTTATCCATATGACTGTTTA
TACCACAAATATTCTTTGAGTTGTCCTCTGAAATAGCTGGTGTTACATTTTTCCAGCTTG
CAGATGTATGGTCTTGCGGTGTAACATTATATGTAATGTTGGTTGGAGCTTATCCATTCG
AGGATCCACAGGAGCCACGAGATTATCGAAAGACAATACAAGTAATGTTTTTCAATTGTT
CTTGCTGTCTCATCATTCATCATTAANANGAAGGAGTAATTACCAATTTTTTTTCAATTC
ACAAATGTA

Last Updated on 06.12.2018 16:11