Sequences from confirmation amplicon


Confirmation sequences for line 056B03 and hit At3g51600 are shown.
Line ID 056B03
Locus At3g51600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19139287
Primers used for PCR BA36 - 8474, show primer details
   
Sequence Name 25-K104830-0022-056-B03C-BA36
GenBank Accession LN497431 [GenBank]
Sequencing primer gene specific primer, BA36: 5'-ACGTACGTGTTACAGTTGGTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K104830-0022-056-B03C-BA36
CAGCACCAGGGAGACGAGCAGCACGACCAGCGTTAAGTCGAGAACCCAAGGCTCTCGCTG
CTCCCTGGATCAACGACAAGCTTGTTGGCGGTCACGGGTGGTACGAGCCAAGCTGTTGAG
CCTCTGAACGCCAGAGCAACACCCTCTAGGAATGAAACCGCCTCGGGTCAAGTAGTTATA
GCATTGACCTAAGCTGCCGGTGACTGCGCCGCACGAGATTGCTGCCTCGGACGCCATTGG
AGCGGTCACTAACATGCACACAATCACCAAAGTTGACAACTTCAAGAGTCCCTCCATATC
TNAATTTTTTTTTTTTCTCTTTTTACTTAGTAAGTAAGGGATTGGATTGGATAATGAAAA
AGGGTAATGAAGGTTGGGTTTATATAGAGAGAGCTTATGAGTGCAAATGCATGAAATTAA
AAGGTGTGAGGTTAGTTGGGGGTTGGTTTCATTTATCTGCAGGAATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTCCCATTTTTTTTTCAATTCAAAAATGTAAATGTCCCC
   
Sequence Name 40-K104831-0022-056-B03C-8409
GenBank Accession LN503699 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104831-0022-056-B03C-8409
CATTTACAATTGAATATATTCCTGCAGATAAATGAAACCAACCACCAACTAACCTCACAC
CTTCTACTTTCATGCATTTGCACTCATAAGCTCTCTCTATATAAACCCAACCTTCATTAC
CATTCTTCATTATCCAATCCAATCCATTACTTACTAAGTAAAAAGAGAAAAAAAAAAAAT
TAAGATATGGAGGGACTCTGGAAGTTGTCAACTTTGGTGATGGTGTGCATGTTAGTGACC
GCTCCAAGGGCGTCCGAGGCAGCAATCTCGGGCGGGGCAGTCACCGGCAGCTTAGGTCAA
GGCTATAACTACTTGACCCGAGGGGGTTTCATTCCTAGAGGGGGTTGCTCGGGCGTTCAA
AGGCTCAACAGCTGGGCTCGTACCACCCGTGACCGCCAACAAGCTTGTCGTTGTATCCGG
GGAGCAGCGAGAGCCTTGGGTTCTCGACTTAACGCTGGTCGGGCTGCTCGTCTCCCGGGG
GCTTGCCGGGTTAGGATCTCTTACCCCATCAGTGCCAAAACCAACT

Last Updated on 06.12.2018 16:11