Sequences from confirmation amplicon


Confirmation sequences for line 393G12 and hit At3g51860 are shown.
Line ID 393G12
Locus At3g51860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19240979
Primers used for PCR RK93 - 8474, show primer details
   
Sequence Name 21-K022407-022-393-G12C-8409
GenBank Accession LN495485 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K022407-022-393-G12C-8409
GTAGATTTCCCGGACATGAAGCCATTTCCATATTTATAAATTCAATTTTCCAAATATGTA
TGTACCCGGTCGAACCGCTGCTCGCGGCGGATATTGGCGATACCACCAAAGAAGAGGGAA
GTGCCAAGAACCAAGAGAAGGTTTGAGAGAATTGAACCCAATAGAGAGTATTTCACCACT
GCCACTTTGTTATTGGCCAACGCTAGTATCGCGATTATCAGCTCTGTCGCGTTTCCACAA
GTCGCGTTCAACAAACCGCCCACTGCCGAAGTAAACATTAATTAGGAGATGTATGTTATT
TGATTCAGAGATCATATTATATGATAAAATAATGTGTTTCGTATGAAAAGGTTACCTGTT
GGACCAGTGTAGAAAGCTAGTTGCCTGCAAGAATAAAAAAGACGATCATGTTCTATTTTC
TTAACAAGGGAGTATAAAATTCGTTTCTAATACAATAACGCTTTTATCAAATTATTTTGT
AGAAAAGTCTAAGTGTGTAACTCTTTTTTCTGTTAAAGAAACAAAAGATTTAGCTATTTA
ACATCGTTTCGAAATTTCAAAAGAATCTTAAAAGTGATGATCATTCAGTCAACACTTGGA
AATGTCAATTACTTTATACTAGTTACTAAACTAAAACAAACTTAACCCAACAC
   
Sequence Name 22-K022407-022-393-G12C-RK93
GenBank Accession LN495933 [GenBank]
Sequencing primer gene specific primer, RK93: 5'-TTTCGACATAAATGTGTTGGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K022407-022-393-G12C-RK93
GTTTGTTTTAGTTTAGTAACTAGTATAAAGTAATTGACATTTCCAAGTGTTGACTGAATG
ATCATCACTTTTAAGATTCTTTTGAAATTTCGAAACGATGTTAAATAGCTAAATCTTTTG
TTTCTTTAACAGAAAAAAGAGTTACACACTTAGACTTTTCTACAAAATAATTTGATAAAA
GCGTTATTGTATTAGAAACGAATTTTATACTCCCTTGTTAAGAAAATAGAACATGATCGT
CTTTTTTATTCTTGCAGGCAACTAGCTTTCTACACTGGTCCAACAGGTAACCTTTTCATA
CGAAACACATTATTTTATCATATAATATGATCTCTGAATCAAATAACATACATCTCCTAA
TTAATGTTTACTTCGGCAGTGGGCGGTTTGTTGAACGCGACTTGTGGAAACGCGACAGAG
CTGATAATCGCGATACTAGCGTTGGCCAATAACAAAGTGGCAGTGGTGAAATACTCTCTA
TTGGGTTCAATTCTCTCAAACCTTCTCTTGGTTCTTGGCACTTCCCTCTTCTTTGGTGGT
ATCGCCAATATCCGCCGCGAGCAGCGGTTCGACCGGGTACATACATATTTGGAAAATTGA
ATTTATAAATATGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG

Last Updated on 06.12.2018 16:11