Sequences from confirmation amplicon


Confirmation sequences for line 447A12 and hit At3g51920 are shown.
Line ID 447A12
Locus At3g51920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19269624
Primers used for PCR BW32 - 8474, show primer details
   
Sequence Name 06-K104337-0022-447-A12C-BW32
GenBank Accession LN488793 [GenBank]
Sequencing primer gene specific primer, BW32: 5'-GATGCTGATTCCTACAACCAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104337-0022-447-A12C-BW32
TTATCATCTTTATATAACTAAACGGTTTTAAGTGTGTATATATATTTTACCTGAGAAGTG
TTTTGAGCCATAATATACAAGAAATCATCAAAAGTGATGCCACCGTTGCCAAAGATGTCA
ACATCGCTCATCATCTGTTGCAGTTGTTCCGCCTTTGGATTCTTCCCCATCGACTTCATC
ACTTTCGTTAGCTTCTCCTTCGTGATGAACCCTGTTTCACATGCATCATAACATCATCAA
ACCGATCGATTCTGAATAAATTTGTGTATCAAATCGAACAAGAAAGAGAGAGAACAAAGC
AGACCATCGGAATCTTTGTCTATGAGACAGAAGGCTTCGTAAAACTCTTGGATCTGTTCA
TCTGTGAAAGCATCCGCCATATCTTCGATCACAAAGAAAAGTCAAAGTCTGTCTTTGATT
CAATCTGATGATCAGCTTCTCTTAATTTGTTAGTGATGAGAGAAGAAGAAGGGAGCATCT
ATCACATTTAAGTACACATCACCTCTTGGCTCTAGGCTCTTGCCCGGTCAAGGTCAATAC
TCATGACCTTGCGTTTTCATTAGTTGTGATATTTTTTCTTGAGCACGATTCCAGGTCACG
CGTCTTTGTTCAAACTTATGACTTCAACGCTTATTTTTCTTTATTTTCATAGTTATTAGA
AAAAATACTTATAGGGAATTAAATTAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 22-K104338-0022-447-A12C-8409
GenBank Accession LN496094 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104338-0022-447-A12C-8409
TTGAATAATCCTAATTTAATTCCCTATAAGTATTTTCTCCTAATAACTATGAAAATAAAG
AAAAATAAGCGTTGAAGTCATAAGTTTGAACAAAGACGCGTGACCTGGAATCGTGCTCAA
GAAAAAATATCACAACTAATGAAAACGCAAGGTCATGAGTATTGACCTTGACCGGGCAAG
AGCCTAGAGCCAAGAGGTGATGTGTACTTAAATGTGATAGATGCTCCCTTCTTCTTCTCT
CATCACTAACAAATTAAGAGAAGCTGATCATCAGATTGAATCAAAGACAGACTTTGACTT
TTCTTTGTGATCGAAGATATGGCGGATGCTTTCACAGATGAACAGATCCAAGAGTTTTAC
GAAGCCTTCTGTCTCATAGACAAAGATTCCGATGGTCTGCTTTGTTCTCTCTCTTTCTTG
TTCGATTTGATACACAAATTTATTCAGAATCGATCGGTTTGATGATGTTATGATGCATGT
GAAACAGGGTTCATCACGAAGGAGAAGCTAACGAAAGTGATGAAGTCGATGGGGAAGAAT
CCAAAGGCGGAACAACTGCAACAGATGATGAGCGATGTTGACATCTTTGGCAACGGTGGC
ATCACTTTTGATGATTTCTTGTATATTATGGCTCAAAACACTTCTCAGGTAAAATATATA
TACACACTTAAAACCGTTTAGTTATAGTAAAGATTGATAATTGTAGAGAAAAGTTAATAA
TGAAAAAATTGGTTGTA

Last Updated on 06.12.2018 16:11