Sequences from confirmation amplicon


Confirmation sequences for line 235E05 and hit At3g52720 are shown.
Line ID 235E05
Locus At3g52720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19540317
Primers used for PCR JL26 - 8474, show primer details
   
Sequence Name 32-K032416-022-235-E05C-8409
GenBank Accession LN500468 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K032416-022-235-E05C-8409
TAATAATTTATTATAAATAAATCAGTCAATAAATTAATAATAATAATAATAATAATAATA
TAATTGGGCCGGGGGCTTACTGGACTCCATGGAGGTGATGTTCAGAAGGAGTGTGCCAAT
GCATTTGCAGTAAGGTATAGTTCTTGTTTTCTATTATCACATCTCCTGCTCCCTCCCCGA
AGAACATGGCAACATTACAGACGTGGTTCACTAGTGTTGCGTTTGTGAAGTAGTATTCAC
GGTGTATTGAATTCAATTTGTGGTTGTAAAATATTTGCCTCCTTTGAATATCAATTGGAG
ATTGCAATTTACCGACCGCGCATGTGGTGAAGTGAGGGTTTAAGTGTCCCCATTGGTTTG
GTCCATTTTTGCCTTTATATCCAAACACTACTCCTTCTGTCTCTGCTCATTTATTACCAA
TTTGAATACGGTTAAAACTTATAAAGTTATATTCACTAAGCACAACACATATCGTTTTAG
ACGGTTAGACCCATACCAAAAACTCTCATATAATAGCACGATTACCATCATTTACTTGAT
CGTTTTAAACTTAATTAAATCCCATCCTATGTATCAAACTTTGAACTTTCTGATTAATTT
ATTGTGTTTTCTCTAAATAAAACTATAAACATGTGAATGCATACTATGAATATAGAAATG
GA
   
Sequence Name 32-K032416-022-235-E05C-JL26
GenBank Accession LN500469 [GenBank]
Sequencing primer gene specific primer, JL26: 5'-TGTTTCTCTCTCCCTCCATTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K032416-022-235-E05C-JL26
AGTTTTATTAGAGAAAAACAATAAATTAATCAGAAAGTTCAAAGTTTGATACATAGGATG
GGATTTAATTAAGTTTAAAACGATCAAGTAAATGATGGTAATCGTGCTATTATATGAGAG
TTTTTGGTATGGGTCTAACCGTCTAAAACGATATGTGTTGTGCTTAGTGAATATAACTTT
ATAAGTTTTAACCGTATTCAAATTGGTAATAAATGAGCAGAGACAGAAGGAGTAGTGTTT
GGATATAAAGGCAAAAATGGACCAAACCAATGGGGACACTTAAACCCTCACTTCACCACA
TGCGCGGTCGGTAAATTGCAATCTCCAATTGATATTCAAAGGAGGCAAATATTTTACAAC
CACAAATTGAATTCAATACACCGTGAATACTACTTCACAAACGCAACACTAGTGAACCAC
GTCTGTAATGTTGCCATGTTCTTCGGGGAGGGAGCAGGAGATGTGATAATAGAAAACAAG
AACTATACCTTACTGCAAATGCATTGGCACACTCCTTCTGAACATCACCTCCATGGAGTC
CAGTAAGCCCCCGGCCCAATTATATTATTATTATTATTATTATTATTAATTTATTGACTG
ATTTATTTATAATAAATTATTATTGTTATAAAATAAAATAAAATTCCAATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATNTCGACGTGTCTACT
Primers used for PCR FP60 - 3144, show primer details
   
Sequence Name 47-K033040-022-235-E05C-3144
GenBank Accession LN506111 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K033040-022-235-E05C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTTT
GATTATCTACCAAATTAGCTAGGTTGGAGCTCAAATAATCGAGTTTTGGATTTTATGCCA
ATTTGTTACTCCACATTTTAAAAACCCCCAATATTATTTTTTATGTCCATAAGAGTCACA
AACTCACAAGCCCATTGGTATGTAAGTGTTTTTTTTGGTGTTTGTCTGGCTTGTTATATA
AGCCCATGTGGATTCTGTCTTCTTTTCTTTTTGAATGTTACTATAAGGTTTTGGAGTGGA
GTTATATATATCATTTGAATCGTTTTTTAAAGTAAAAAAAAGTGTTAATTAATGATAATA
ATTGAACAAAAACGTAATTATGCA
   
Sequence Name 15-K033040-022-235-E05C-FP60
GenBank Accession LN492868 [GenBank]
Sequencing primer gene specific primer, FP60: 5'-GCTGCATATCTACGTTTTTGTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K033040-022-235-E05C-FP60
ATACCTTTTTTTACTTTAAAAAACGATTCAATGATATATATAACTCCACTCCAAAACCTT
ATAGTAACATTCAAAAAGAAAAGAAGACAGAATCCACNATGGGCTTATATAACAAGCCAG
ACAAACACCAAAAAAAACACTTACATACCAATGGGCTTGTGAGTTTGTGACTCTTATGGA
CATAAAAAATAATATTGGGGGTTTTTAAAATGTGGAGTAACAAATTGGCATAAAATCCAA
AACTCGATTATTTGAGCTCCAACCTAGCTAATTTGGTAGATAATCAAACAAACACTGATA
GTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATG
AAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATC
CCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTC
TTCTTTTTCCACGATGCTCCTCGTGGGTGG

Last Updated on 06.12.2018 16:11