Sequences from confirmation amplicon


Confirmation sequences for line 361H01 and hit At3g52720 are shown.
Line ID 361H01
Locus At3g52720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19540279
Primers used for PCR DR22 - 8474, show primer details
   
Sequence Name 05-K026766-022-361-H01C-8409
GenBank Accession LN488204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K026766-022-361-H01C-8409
CCGGACATGAAGCCATTTACAATTGAATAGCCAAATAATCGAGTTTTGGATTTTATGCCA
ATTTGTTACTCCACATTTTAAAAACCCCCAATATTATTTTTTATGTCCATAAGAGTCACA
AACTCACAAGCCCATTGGTATGTAAGTGTTTTTTTTGGTGTTTGTCTGGCTTGTTATATA
AGCCCATGTGGATTCTGTCTTCTTTTCTTTTTGAATGTTACTATAAGGTTTTGGAGTGGA
GTTATATATATCATTTGAATCGTTTTTTAAAGTAAAAAAAAGTGTTAATTAATGATAATA
ATTGAACAAAAACGTAAATATGCAGCTGAACTGCACATGGTACACCAAGCAAAAGATGGA
AGCTTTGCTGTGGTGGCAAGTCTCTTCAAAATCGGCACTGAAGAGCCTTTCCTCTCTCAG
GTACTTTTGAGAAGTGTGACTATAGATTGGGATAATTTGATAATTCAATGTTATTAATAT
AATGTGAAAATATGA
   
Sequence Name 06-K026766-022-361-H01C-DR22
GenBank Accession LN488655 [GenBank]
Sequencing primer gene specific primer, DR22: 5'-TCGATTCTTCCTACTTCCACTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K026766-022-361-H01C-DR22
GTGGTTCCCTTTGAGTCTCTCTTCCTTTAGCTTCACCAATTTCTCCTTCATCTAATCATA
TTTTCACATTATATTAATAACATTGAATTATCAAATTATACCAATCTATAGTCACACTTC
TCAAAAGTACCTGAGAGAGGAAAGGCTCTTCAGTGCCGATTTTGAAGAGACTTGCCACCA
CAGCAAAGCTTCCATCTTTTGCTTGGTGTACCATGTGCAGCTCACCTGCATATCTACGTT
TTTGTTCAATTATTATCATTAATTAACACTTTTTTTTACTTTAAAAAACGATTCAAATGA
TATATATAACTCCCCTCCAAAACCTTATAGTAACTTTCAAAAAGAAAAGA

Last Updated on 06.12.2018 16:11