Sequences from confirmation amplicon


Confirmation sequences for line 180C10 and hit At3g53680 are shown.
Line ID 180C10
Locus At3g53680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19894116
Primers used for PCR 10gp - 8474, show primer details
   
Sequence Name 53-K112131-0022-180-C10C-10gp
GenBank Accession LN508196 [GenBank]
Sequencing primer gene specific primer, 10gp: 5'-AAACATGTTGGAAGAATTACCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K112131-0022-180-C10C-10gp
GATGAAAGGACTTGAATCTGAGTTTATCATAGTACAGCTTACCCATAGACCATGACAGGA
ATGAGATCACGACCAGACTTTGCCACAATGGGATCAAAACACTCCTGAAAAGTAAACTCA
TTTGCTATACATGTGTTTATAAAATAAATTCGCTGTACAATGTACATGTGTGTAAGAAAT
AAATTTACAAGCCAGATAAAACTCAAAATGATGTGAGCACATTTATAAATTGAGGCATAC
CCTGAAAATAGTAGCTGCTCGTGAAAGTAAGGGTAGATGCTCTGGGTATCGACTTTTTCC
ACTAAGCATCCTCCACTCCACAGTATTTCCATTGTCAATATATATTCCTTTTTCTCTGTA
CTTTCTGCTTATCGTGTCTAACAAAAGCGTGGGAATAGTTTGTGGCCCACATGAAGCAGA
GCTTTGAAGAACCCTGTGAATCCTGCTGCAGTCACTACAGCAGAACCATTTATCTACATG
TGAACAATCAACAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCC
   
Sequence Name 37-K112130-0022-180-C10C-8409
GenBank Accession LN502662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112130-0022-180-C10C-8409
GCATTTACTTTGTTGATTGTTCACATGTAGATAAATGGTTCTGCTGTAGTGACTGCAGCA
GGATTCACAGGGTTCTTCAAAGCTCTGCTTCATGTGGGCCACAAACTATTCCCACGCTTT
TGTTAGACACGATAAGCAGAAAGTACAGAGAAAAAGGAATATATATTGACAATGGAAATA
CTGTGGAGTGGAGGATGCTTAGTGGAAAAAGTCGATACCCAGAGCATCTACCCTTACTTT
CACGAGCAGCTACTATTTTCAGGGTATGCCTCAATTTATAAATGTGCTCACATCATTTTG
AGTTTTATCTGGCTTGTAAATTTATTTCTTACACACATGTACATTGTACAGCGAATTTAT
TTTATAAACACATGTATAGCAAATGAGTTTACTTTTCAGGAGTGTTTTGATCCCATTGTG
GCAAAGTCTGGTCGTGATCTCATTCCTGTCATGGTCTATGGGTAAGCTGTACTATGATAA
ACTCAGATTCAAGTTCCTTTTTCATTCAAATGTTAATAGTAATTGATCATCCTGGTAATT
C

Last Updated on 06.12.2018 16:11