Sequences from confirmation amplicon


Confirmation sequences for line 786D04 and hit At3g57090 are shown.
Line ID 786D04
Locus At3g57090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21128749
Primers used for PCR JL31 - 8474, show primer details
   
Sequence Name 06-K032516-022-786-D04C-8409
GenBank Accession LN488693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K032516-022-786-D04C-8409
ATTTACATTGAAGGAATAGAGAAAACTACATGGCAAAACTTACAGCGATGACATCTCCGT
CGCACCATGGGATCTTATCGCTGCCGCTGAAGAAAGTACCGACGGAGTCAAAGAATTGTC
CGATCTTAGCATCCATTGAAGGCGATTTTGAGCTTTGATTGATGAGGTTTTCGTTTCTGG
TCTCTTTCTTAATTCTTAAGGAGAGTTTCTTAAGGAGAGAAGTTGGACTTGTTGCCCAAG
CTCAGGGAAACAGACGATATTTATCCCTTGAATTTATCAACAAGACCCCTGTATTGTTAA
GACTTACCCACATTGATCACCATATCTTCTG
   
Sequence Name 06-K032516-022-786-D04C-JL31
GenBank Accession LN488694 [GenBank]
Sequencing primer gene specific primer, JL31: 5'-CAGAAGATATGGTGATCAATGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K032516-022-786-D04C-JL31
TGATAATTCAGGGATAAATATCGTCTGTTTCCCTGAGCTTGGGCAACAAGTCCAACTTCT
CTCCTTAAGAAACTCTCCTTAAGAATTAAGAAAGAGACCAGAAACGAAAACCTCATCAAT
CAAAGCTCAAAATCGCCTTCAATGGATGCTAAGATCGGACAATTCTTTGACTCCGTCGGT
ACTTTCTTCAGCGGCAGCGATAAGATCCCATGGTGCGACGGAGATGTCATCGCTGTAAGT
TTTGCCATGTAGTTTTCTCTATATCCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAATGG
Primers used for PCR FQ45 - 8474, show primer details
   
Sequence Name 61-K118414-0022-786-D04C-FQ45
GenBank Accession LN510622 [GenBank]
Sequencing primer gene specific primer, FQ45: 5'-TAGCAGATGAAGCTAGACGATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K118414-0022-786-D04C-FQ45
AACAAAACCATTGATGCTGTGACATTTGGTAAAGTTCTACACAGCTATCAAATTCGGATC
CAACATAAGGGTGCAAAAATCAGTAATCACGCTCATTTGAGGACGAATTTTACAGCAACT
CATGAACTTCTAAAGCTCGATTCAATCGAATCCAATAAAAAAATATCCTCGAGAAAGCAA
ATCCGATTTCAGAAATTTCCAAGCAGAAACACGAAAACAGGTCTAAATTTCTGCAAATGG
TTCATGATTCCATTTGCCTACAAGAGATGGTTCATCTTCTTCGGAAACATAGGCGGAAAA
AAGCGATATAGAGTATGATGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACGGTTATTATAAA
   
Sequence Name 13-K118413-0022-786-D04C-8409
GenBank Accession LN492255 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118413-0022-786-D04C-8409
CATTTCATCATACTCTATATCGCTTTTTTCCGCCTATGTTTCCGAAGACGATGAACCATC
TCTTGTAGGCAAATGGAATCATGAACCATTCGCAGAAATTTAGACCTGTTTTCGTGTTTC
TGCTTGGAAATTTCTGAAATCGGATTTGCTTTCTCGAGGATATTTTTTTATTGGATTCGA
TTGAATCGAGCTTTAGAAGTTCATGAGTTGCTGTAAAATTCGTCCTCAAATGAGCGTGAT
TACTGATTTTTGCACCCTTATGTTGGATCCGAATTTGATAGCTGTGTAGAACTTTACCAA
ATGTCACAGCATCAATGGTTTTTTGTTTGATAGCTTTGTATCTTCTTCAATCGTCTAGCT
CCATCTGCTA

Last Updated on 06.12.2018 16:11