Sequences from confirmation amplicon


Confirmation sequences for line 562A03 and hit At3g58450 are shown.
Line ID 562A03
Locus At3g58450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21621973
Primers used for PCR 21eq - 8474, show primer details
   
Sequence Name 21-K110827-0022-562-A03C-21eq
GenBank Accession LN495712 [GenBank]
Sequencing primer gene specific primer, 21eq: 5'-GTACTCTCTTCTCGTGCTTTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K110827-0022-562-A03C-21eq
ACCGCCGAAGCTAGAATTTCCAACTCACTCAATATTGTATACAGTCACATCCCATATAAT
ATATATATACGCATAGAGAATAAAAGAAAGAGAGAAGCGTTTTTTTCTTCACCAGTTCCA
CCAGAAGGATAGATATATTGAAGGTACGTTGGGTGAACATGAAGCAACGTAAGTAAACCG
CCTTCTTGACCGGTTTCCGGTTCGGCACTAATAACAACTCTGAGATGATCCACAGCCCAT
TCCAACGCATCGAAGCTGTTCTTGCTCTCATCAATCGCAACCATCACTTTCAACTTCTTC
TTGTTCTTATTTGCAGCCGTCTCCGCCGTCGTTGTTGTGGCTGCCGTGTCCTCTCCAATC
GCATCAACGTAAGTCTCCATTTTTTACTCCGTACGTTTTCTCTATCGTATGTTTTTTTTG
TTCGGCTCCAGCCACTTTTTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAACATTATT
   
Sequence Name 05-K110826-0022-562-A03C-8409
GenBank Accession LN488410 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K110826-0022-562-A03C-8409
TTGAATATTCAAAAGTGGCTGGAGCCGAACAAAAAAACATATGATAGAGAAAACGTACGG
AGTAAAAAATGGAGACTTACGTTGATGCGATTGGAGAGGACACGGCAGCCACAACAACGA
CGGCGGAGACGGCTGCAAATAAGAACAAGAAGAAGTTGAAAGTGATGGTTGCGATTGATG
AGAGCAAGAACAGCTTCGATGCGTTGGAATGGGCTGTGGATCATCTCAGAGTTGTTATTA
GTGCCGAACCGGAAACCGGTCAAGAAGGCGGTTTACTTACGTTGCTTCATGTTCACCCAA
CGTACCTTCAATATATCTATCCTTCTGGTGGAACTGGTGAAGAAAAAAACGCTTCTCTCT
TTCTTTTATTCTCTATGCGTATATATATATTATATGGGATGTGACTGTATACAATATTGA
GTGAGTTTGGAAATTCTTAGCTTCGGCGGTGTATGCGACAGATTCAGTGCCCGAACCAAT
GAGGAAAGCACGAGAAGAGAGTA

Last Updated on 06.12.2018 16:11