Sequences from confirmation amplicon


Confirmation sequences for line 450D05 and hit At3g59020 are shown.
Line ID 450D05
Locus At3g59020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21815031
Primers used for PCR EP70 - 8474, show primer details
   
Sequence Name 34-K103008-0022-450-D05C-8409
GenBank Accession LN501373 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103008-0022-450-D05C-8409
GCCTTTACATTGAATATACATGGGGACATCACGTGCAATGATTTTTCCAGTTTAATTGAT
CCAGAGAACTCATATTCAGATAAATTTCTAAAATTGATCAAAAGTAGGCCATATAGAACA
TCCGACCTGTTGCTTAGATATTGAAGAATTAGGTTGATGACTCTATCCGGAAGATATCCA
CCTATACGAATGGCATTCAACAATTTCAGGTGGCACTCTAATATCTTGGCTGCATAGTTT
ATCTGGAACATTTGAGCAAAGGCTTTGTTATCTGGATTCTGGAGTTTCAGATCTCCAAAC
CTATAACAAGTTTTCAAAACTGTTATTTAAGGTAGATGAACAAATGCTAGGTTATAAGTT
AACGGTAAGATTTTCATGTCCCCGCATACCGAGTGTATAGCCTGTTTAAGATATGAGCAA
TCCACTTCTTGGCCTTCCACCATCCCCAGGATTTTCTAAGCTCAGGGTCTTCAGGCTGGC
CTTCTACGGGAACAGGACGTTCCAAAATGTTTA
   
Sequence Name 82-K103009-0022-450-D05C-EP70
GenBank Accession LN514179 [GenBank]
Sequencing primer gene specific primer, EP70: 5'-CTAAACATTTTGGAACGTCCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K103009-0022-450-D05C-EP70
AAATCCTGGGGATGGTGGAAGGCCAGAAGTGGATTGCTCATATCTTAAACAGGCTATACA
CTCGGTATGCGGGGACATGAAAATCTTACCGTTAACTTATAACCTAGCATTTGTTCATCT
ACCTTAAATAACAGTTTTGAAAACTTGTTATAGGTTTGGAGATCTGAAACTCCAGAATCC
AGATAACAAAGCCTTTGCTCAAATGTTCCAGATAAACTATGCAGCCAAGATATTAGAGTG
CCACCTGAAATTGTTGAATGCCATTCGTATAGGTGGATATCTTCCGGATAGAGTCATCAA
CCTAATTCTTCAATATCTAAGCAACAGGTCGGATGTTCTATATGGCCTACTTTTGATCAA
TTTTAGAAATTTATCTGAATATGAGTTCTCTGGATCAATTAAACTGGAAAAATCATTGCA
CGTGATGTCCCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11