Sequences from confirmation amplicon


Confirmation sequences for line 476F03 and hit At3g59650 are shown.
Line ID 476F03
Locus At3g59650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22033101
Primers used for PCR HF52 - 8474, show primer details
   
Sequence Name 28-K101325-0022-476-F03C-8409
GenBank Accession LN498770 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K101325-0022-476-F03C-8409
ATTTACAATTGAAGCTTTTAGTCTGTAACATAAGTCACGTGCCCTTTAACGTGATCATCT
TCTTTCTTCCTTTTTTCTTGTCCTCTGTTACACATCGGTTAGGGTTTTAGTTTCTCTTAC
CGAGATTCAAATGGCGCTTAGAGGAGTTTGGCAGCTCAAGAAACTCGTTGTGAGCTACTG
TAATTGGGGTGGTAGCAGTAGAGGCATCAGGTAATTTTGTTTACCTATAATTTGATTTCG
CAATCTATGAGCCGTATTTTTTCATTTATCATCGATCTACCATTTTTCCTGTAATTCACT
TTGGAACTTGAGACTATCTATACGAATCTATGAGTCTGTACTTGGTATTTGTGAAGCAAA
TTAACTTTTCATGTCAATGTGGGTTTAGAAATGTGAAGCAAAATGATTTAGTAATGAATC
GTTACCATTTCATCCTAGTTTGGTACTACGCATTTTCTTATGATGTGTTGTTAAACATAC
AGAGCCTTTATGGAATCAGAATTGCCTGCTCTAAAGGAGAAAAACCCGCAGCTCGAAGTA
ATTACCGAGCTTTCACGGGGACAACATCCTTATTTGAAGGGCATTTACAGTATGTATATT
TCTCCTCTATTGAATACAATCCTGATTCCACGGTTGTTGATATTAGCAATTTCACTTGCA
CAATTCANAAGGAATAAAAATGAAAGGGTAGTGTGTGTGAAGAACATGGATCCTGAAGAA
ATGCTTTTGAATGCAACGAGGCTGAAGAACTCGCTTGGACGGAAAGTGGTTAAACTGA
   
Sequence Name 28-K101331-0022-476-F03C-HF52
GenBank Accession LN498771 [GenBank]
Sequencing primer gene specific primer, HF52: 5'-GTGACATGTCTGGTCCTCAGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K101331-0022-476-F03C-HF52
CTCACCTCGTTGATTCAAAGCACTTCTTCAGGATCCATGTTCTTCACACACACTACCCTT
TCATTTCTATTCCCTATGAATTGTGCAAGTGAAATTGCTAATATCAACAACCGTTGAATC
AGGATTGTATTCAATAGAGGAGAAATATACATACTGTAAATGCCCTTCAAATAAGGATGT
TGTCCCCGTGAAAGCTCGGTAATTACTTCGAGCTGCGGGTTTTTCTCCTTTAGAGCAGGC
AATTCTGATTCCATAAAGGCTCTGTATGTTTAACAACACATCATAAGAAAATGCGTAGTA
CCAAACTAGGATGAAATGGTAACGATTCATTACTAAATCATTTTGCTTCACATTTCTAAA
CCCACATTGACATGAAAAGTTAATTTGCTTCACAAATACCAAGTACAGACTCATAGATTC
GTATAGATAGTCTCAAGTTCCAAAGTGAATTACAGGAAAAATGGTAGATCGATGATNAAT
GAAAAAATACGGCTCATAGATGGCGAAATCAAATTATAGGTAAACAAAATTACCTGATGC
CTCTACTGCTACCACCCCCATTACAGTAGCTCACAACGAGTTTCTTGAGCTGCCAAACTC
CTCTTAGCGCCATTTGAATCTTGGTTAGAGAAACTAAAACCCTAACCGATGTGTTACAGA
TGACCAGAAAAAAGGAAGAAAGAAGATGATCACGTGATAAGTGCACGTGACTTATGTTAC
AGACCAAATAGCTTCAATTGTAAATGGCTTCATGTCCCGGAAATC

Last Updated on 06.12.2018 16:11