Sequences from confirmation amplicon


Confirmation sequences for line 942D02 and hit At3g59650 are shown.
Line ID 942D02
Locus At3g59650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22033860
Primers used for PCR 28yj - 8474, show primer details
   
Sequence Name 89-K119982-0022-942-D02C-28yj
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 28yj: 5'-CACCAGATAAGAAAGTGCTGGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K119982-0022-942-D02C-28yj
TGTTGTTTAGGCAAATGAAATAAGAATTATCTGTCTGATAATACTTGTCTTCCCGTCTTT
CAGGTTTTTGAAACAGTGCAACAGCCACATGATGTTCCTTTTGGTTCTTACTTTGAGGTA
TGTTACCTATGAGGAAAATGTGGTTCTTGTTTCATGATTCTTTTTGCAACCCACCAATCA
AATTTTACCTTGGCATGCTTTATAAATTCTGTTTGTATACCAGGTCCACTGTCGATGGCG
CTTGGAAGCTAAAGACGAGACTTCATCTGTTATAGATATAAGAGTCGGTAAGTAAACCAA
AGTAATGATTTCTACTTTTCCGTGTGAAATTTTGCAAAGCACGTTGTGTGGAATCTCTTT
GGCTTCCTCAATAGGTGTACATTTCAAGAAATGGTGTTTGATGCAATCGAAGATTAAAGC
AGGGGCAATTGACGAGGTTAGTTTCAATCGATAAACTAAAAGGCCGCCATTTTCGGTTTG
TCTGATGCATTTGTTTTTGACACTTTCTTTACACAGTACAAGAAAGAAGTGGAAGTGATG
CTAGAAGTGGCATTGTCGTATCTCAAGTCACACTCTTCCTCAAGTAGCCATGGTGATATA
GACAAGTCTGCTCTATCATCTCCAACACCAGAAAACATCAGCTAAAACCTAATTTACCAA
AATCAAAAAGCTTCTCAAAGTTTCATTCTCCAGCCAGCTAATTAAGATCATCCACCATCA
TTTTTCTTCTTCTTTTACATGTTAAAGTGTATATGTGGTAACAGTAATTATGAATGGATA
TTTACAGAGGCTTGTCTGATTTCTGGTACTTGTGACAATAACAAAGGAAAAGTTTGGGTT
CTCTCACAACTCTTATTGTATTGGCAAATACCAAGTAAGTGAAATTTCCATTC
   
Sequence Name 73-K119981-0022-942-D02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K119981-0022-942-D02C-8409
CATTTACATTGAATATATCCTGAGGACCAGACATGTCACCAAACACCCCACCTGTTCAAG
GCACCTGGACAACTGCTGTCAAATTCTGAGACTTTCCTGCTAAATTGTTTGCAGGTTTTC
ATCTTCTAGCTGTTAAATCGAATGTTGCAAGTATCAGGATTGGTCGTTTATCTGTATTGC
AACCCCAAGTTGTTTGTACCGTCTGTGACTTGGGACAAAGATGTAGTCTTCAAATGTTTT
GCAGTTTTTGCAATGTTTGAATTTTGTCTTGTCCTTCTGGTTGAGTCTATAATAAGATTA
CACAAGGGTTTAAGGTTATATTCTTTGTTTGCTCTCAGAATTGGAATTGTCACTTACTTT
GTATTTGCCAATACAAATAGAGTTGTGAGAGAACCCAAACTTTTCCTTTGTTATTGTCAC
AAGTACCAGAAATCAGACAAGCCTCTGTAAATATCCATTCATAATTACTGTTACCACATA
TACACTTTAACATGTAAAAGAAGAAGAAAAATGATGGTGGATGATCTTAATTAGCTGGCT
GGAGAATGAAACTTTGAGAAGCTTTTTGATTTTGGTAAATTAGGTTTTAGCTGATGTTTT
CTGGTGTTGGAGATGATAGAGCAGACTTGTCTATATCACCATGGCTACTTGAGGAAGAGT
GTGACTTGAGATACGACAATGCCACTTCTAGCATCACTTCCACTTCTTTCTTGTACTGTG
TAAAGAAAGTGTCAAAAACAAATGCATCAGACAAACCGAAAATGGCGGCCTTTTAGTTTA
TCGATTGAAACTAACCTCGTCAATTGCCCCTGCTTTAACCTCGATTGCATCA

Last Updated on 06.12.2018 16:11