Sequences from confirmation amplicon


Confirmation sequences for line 004D11 and hit At3g60770 are shown.
Line ID 004D11
Locus At3g60770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22460381
Primers used for PCR 01oz - 8474, show primer details
   
Sequence Name 20-K114895-0022-004-D11C-01oz
GenBank Accession LN495330 [GenBank]
Sequencing primer gene specific primer, 01oz: 5'-CTGTCGAGAGAAGGAGAACAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114895-0022-004-D11C-01oz
GAAAGCACGACTTGTAACTTCTCGGACACTGATACCCATTCACAACCGACACGATTGTGT
TATGACACCGTTAGACTTTTACATACACACTTAATTATAGGAGTATAACATAAAACGGAA
ATTCTATTACAAACTAACACTTACAAGCTTACAAATTTTTTTATGTCTTCCATTAACATG
TCCATCCCATCTAGGTGAAGGATCATCAAGAACTATACTTCTATAGGATTCCTTCATTTC
CAAGGTATTAGCCAATCTGATGATTACAAAAACCACAAGAATGCCTGCGCTTGTAGACTC
GGAAATTTGTTTTATCATCGTCGGAGTTTTAGAGTCTTTGTCTAATTTGCTAAACAATAA
GTTAAAAGGAACAGACATTAAAAACATGCAACTGAAAGAAGTATTAAGACAATATCTAAG
CCCAACACTAAATTATCTTCAACTTGGACGCTACTAGCGGAGATAATCTGTAAAAATTCT
CTGAGAACGATNATTAACGTTAATTGAAAGATAAGCTGTAACGATNATGGAAAGATTCGC
ATAAACAAATTCGTTCAAGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATT
   
Sequence Name 08-K114894-0022-004-D11C-8409
GenBank Accession LN489844 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114894-0022-004-D11C-8409
TTCTCGAACGAATTTGTTTATGCGAATCTTTCCATTATCGTTACAGCTTATCTTTCAATT
AACGTTAATTATCGTTCTCAGAGAATTTTTACAGATTATCTCCGCTAGTAGCGTCCAAGT
TGAAGATAATTTAGTGTTGGGCTTAGATATTGTCTTAATACTTCTTTCAGTTGCATGTTT
TTAATGTCTGTTCCTTTTAACTTATTGTTTAGCAAATTAGACAAAGACTCTAAAACTCCG
ACGATGATAAAACAAATTTCCGAGTCTACAAGCGCAGGCATTCTTGTGGTTTTTGTAATC
ATCAGATTGGCTAATACCTTGGAAATGAAGGAATCCTATAGAAGTATAGTTCTTGATGAT
CCTTCACCTAGATGGGATGGACATGTTAATGGAAGACATAAAAAAATTTGTAAGCTTGTA
AGTGTTAGTTTGTAATAGAATTTCCGTTTTATGTTATACTCCTATAATTAAGTGTGTATG
TAAAAGTCTAACGGTGTCATAACACAATCGTGTCGGTTGTGAATGGGTATCAGTGTCCGA
GAAGTTACAAGTCGTTGCTTTCGTGTCTCAGTGAAATAAAAACAAGTTCCCACTTACTTT
GTTCT

Last Updated on 06.12.2018 16:11