Sequences from confirmation amplicon


Confirmation sequences for line 191G09 and hit At3g61360 are shown.
Line ID 191G09
Locus At3g61360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22706151
Primers used for PCR CE04 - 3144, show primer details
   
Sequence Name 50-K105791-0022-191-G09C-CE04
GenBank Accession LN507171 [GenBank]
Sequencing primer gene specific primer, CE04: 5'-GCACACATAAAGCAAAAATCTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105791-0022-191-G09C-CE04
TTGCCTTGAGGTTGAATTTACCAGTTTGAGGATGCGGGCTTCGTGCAGCTCCTTGTTTAG
GCTAAACCTAATATCTAACGCGAGAATACCCGTAAAAACATTTAAAGATAAAAACCCACT
AAAACTCTCCCCACAGACACTATGTGACACAATTTCATCACAAATGGAATCATTAGATTT
TCACAGACAAATCAACAAACACCTTCCGTGCATATACAAAGTGAACCAAACTTTGAGCTA
GAGAACCGCAAGTAAATTCATACACGAACAAGAACTCACATATCTTTGCGTGGATTAATG
AGAAATTACCAGTTCAAAACATGAAATTTGCGTCGCTGCCAAACGAACTCCTTCCTTCTC
TACAGATGCCGATGAATAAGCTCGCGGAGCCGTCCAACAAGTTTTAATAACCAAAATAAA
CCGATGTTCGATTTCAGTTCCTTCGGCATAATTTCGGTTCGGTTCGCTTCGGTTAACTGA
AATGGGCTCCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTA
Primers used for PCR CE03 - 8474, show primer details
   
Sequence Name 51-K105791-0022-191-G09C-CE03
GenBank Accession LN507505 [GenBank]
Sequencing primer gene specific primer, CE03: 5'-GTATGCTCATGGACTTGAAACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K105791-0022-191-G09C-CE03
CCTTCCGCCATTAAGCCCAAGCTTGATCGAATAACGCATCCGTGCGAGAACGTGAAGTGT
TTTCTCGAAGGAATCAGAGGTGGGAGAAGATTTAGAGCTTTTAAGTGAATACTTGAAGAA
TTCGAGAGCTTTAAGGCCATTGGAATGAGCAGCGAAGAGACGACCGAGAACTTCTGATAC
AAATTCCGGTGACAGAGAAGATAAAGGGATGTGTTTGGCGAGAATGGGTTGAATCGGATG
ATTTGGGAATGGATGACCATTGATGATTATTGTAATACGTTCGATCTCTGTTCTGTTTAT
CGATGTAGATGATAAAGATGAAGATGATGATAGTACTGGAGCAAGAAGACGACGATACGA
ATCGGAGGAGATCTTCTGAAGCATTTTTCTCTCTACCAGAAAATTAGATGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATT
   
Sequence Name 59-K105794-0022-191-G09C-8409
GenBank Accession LN510005 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105794-0022-191-G09C-8409
GAGCCTTTACATTGAATATATCCATCTAATTTTCTGGTAGAGAGAAAAATGCTTCAGAAG
ATCTCCTCCGATTCGTATCGTCGTCTTCTTGCTCCAGTACTATCATCATCTTCATCTTTA
TCATCTACATCGATAAACAGAACAGAGATCGAACGTATTACAATAATCATCAATGGTCAT
CCATTCCCAAATCATCCGATTCAACCCATTCTCGCCAAACACATCCCTTTATCTTCTCTG
TCACCGGAATTTGTATCAGAAGTTCTCGGTCGTCTCTTCGCTGCTCATTCCAATGGCCTT
AAAGCTCTCGAATTCTTCAAGTATTCACTTAAAAGCTCTAAATCTTCTCCCACCTCTGAT
TCCTTCGAGAAAACACTTCACATTCTCGCACGGATGCGTTATTTCGATCAAGCTTGGGCT
TTAATGGCGGAAGTGCGAAAAGACTACCCTAATTTGTTGAGTTTCAAGTCCATGAGCATA

Last Updated on 06.12.2018 16:11