Sequences from confirmation amplicon


Confirmation sequences for line 009E06 and hit At4g00840 are shown.
Line ID 009E06
Locus At4g00840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:355636
Primers used for PCR BG63 - 8474, show primer details
   
Sequence Name 05-K104021-0022-009-E06C-BG63
GenBank Accession LN488327 [GenBank]
Sequencing primer gene specific primer, BG63: 5'-TCACTGGGTCTCATCACATAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104021-0022-009-E06C-BG63
AACGACTAGACTTTCACAGAAGCAAAGAAATGAATCAAATCAATTACAATAATACAACAA
CAATTGGCAATGAGAATTCTTTTTGAGTAGAGAGTTATCTACATTGGAGTGCAGTTGAAG
GCTGTTTCGAATATGATTGATTGATTTTGTTTCAACTTTCAATCAAGGGTCGATGTCAGA
ACAAGTTGGAAATTCAAGGCCTTCAAGGGAAGTTATGTTATCGATATCGTCTTTAGAGTA
CAATGGGAGGAGCCAAAACGCCTTCTTCTTCCCAAAAACCTTCACAAGATGAAAACAAAG
AGTTATATATTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCA
   
Sequence Name 21-K104022-0022-009-E06C-8409
GenBank Accession LN495633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104022-0022-009-E06C-8409
TTACAATTAAATATATAACTCTTTGTTTTCTCTTGTGAAGGTTTTTGGGAAGAAGAAGGC
GTTTTGGCTCCTCCCATTGTACTCTAAAGACGATATCGATAACATAACTTCCCTTGAAGG
CCTTGAATTTCCAACTTGTTCTGACATCGACCCTTGATTGAAAGTTGAAACAAAATCAAT
CAATCATATTCGAAACAGCCTTCAACTGCACTCCAATGTAGATAACTCTCTACTCAAAAA
GAATTCTCATTGCCAATTGTTGTTGTATTATTGTAATTGATTTGATTCATTTCTTTGCTT
CTTGTTGAAAGTCTAAGTTCGTTTGACATCTTCAGACTGTAATGTTATGTGATGAGACCC
AGTGA
Primers used for PCR CB42 - 3144, show primer details
   
Sequence Name 24-K105032-0022-009-E06C-CB42
GenBank Accession LN496985 [GenBank]
Sequencing primer gene specific primer, CB42: 5'-TGTTGGATGTTATTGTCTTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105032-0022-009-E06C-CB42
CATTCTTCTTCTCCGGGTAACTGGCCTCTCTCGTTCTGGCATTCGGTGAGTATAAACGGT
TCTTTTTCTCGTTTTCCTTTTCCCAGAAAGTGCTTAGCTTTTTTCTGGGTCTCATTTTCC
GTCTTTATGTTTCTTACAGATTCTATCCCCTTGATGCAGTTCTTAACTTTGCGTTCGTTC
TCAGCCTTCTTTGTTTCGTAGTCATGCATATTTCTCTTCTCTCATCCAACACTACTTCAG
TTGAGGTAAATCTTTTATGTTCTTGCTTTCACAAACAATCTAAGTCTTGAAAATATGAGT
CTCTTCTCTGAATCATTGTCTTATTTTTTTAGGTTCATGAGAAAAATGGAGAAGTCAGAT
GGAAATATGACTTAGGAAAGAAGAAAAACTTCGAGCAGGTGAGATTTTATTGTTTTCATC
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTGAT
TTCGCCATTCCCAGATACCCATTTCATCTTCAGATTGGTCTGAGATTATGCGAAAATATA
CACTCATATACATAAATACTGACAGTTTGAGCTACCAATTCAGTGTAGCCCATTACCTTA
CATAATTCACTCAAATGCTAGGCAGTCTGTCAACTCGGCGTCAATTTGTCGGCCACTATA
CGATAGTTGCGCAAATTTTCAAAGTCCTGGCCTAACATCACACCTCTGTC

Last Updated on 06.12.2018 16:11