Sequences from confirmation amplicon


Confirmation sequences for line 362E04 and hit At4g01360 are shown.
Line ID 362E04
Locus At4g01360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:566164
Primers used for PCR BJ08 - 8474, show primer details
   
Sequence Name 66-K115895-0022-362-E04C-BJ08
GenBank Accession LN511681 [GenBank]
Sequencing primer gene specific primer, BJ08: 5'-TATAACCATGTTGAAGTTTGGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K115895-0022-362-E04C-BJ08
TGGTTGCTTTGATGATTGATCATGTAACCATGAAAATAACACTGATTTTTTTTGGTCTAG
TGATTTACAATGGGCCTCAAGCAAACCCATCACTTATCCATTATCAACTAAGATGAACTA
AAGCACAATTCTGACTAACTTGAAAATGTAGTTATAGCTTAGCACCGATTGAAAAGAGAG
AGTTATCAATATTTGAAGCGTTTTAAAGTCTCATTTTTATCTGCATTTGTTATTACTTAA
CATCAACGATACCATAATTACATGTTTCCTCTATATTCACAAACAAGTCTTAGCTCTTTT
CCTCTATATTAACACTTTTCGTCCACAGGCTCTCCAACAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 36-K115894-0022-362-E04C-8409
GenBank Accession LN502357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115894-0022-362-E04C-8409
CATTTACATTGTTGGAGAGCCTGTGGACGAAAAGTGTTAATATAGAGGAAAAGAGCTAAG
ACTTGTTTGTGAATATAGAGGAAACATGTAATTATGGTATCGTTGATGTTAAGTAATAAC
AAATGCAGATAAAAATGAGACTTTAAAACGCTTCAAATATTGATAACTCTCTCTTTTCAA
TCGGTGCTAAGCTATAACTACATTTTCAAGTTAGTCAGAATTGTGCTTTAGTTCATCTTA
GTTGATAATGGATAAGTGATGGGTTTGCTTGAGGCCCATTGTAAATCACTAGACCAAAAA
AAATCAGTGTTATTTTCATGGTTACATGATCAATCAATCAAAGCAAACCAGATATTCTAG
CTATTAATAATAACAAATGCACCAAACTTCAACATGGTTAT

Last Updated on 06.12.2018 16:11