Sequences from confirmation amplicon


Confirmation sequences for line 343H06 and hit At4g03020 are shown.
Line ID 343H06
Locus At4g03020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1333948
Primers used for PCR GD62 - 8474, show primer details
   
Sequence Name 79-K033710-022-343-H06C-GD62
GenBank Accession LN513772 [GenBank]
Sequencing primer gene specific primer, GD62: 5'-CGTAAACCTGCAATGTAGCAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K033710-022-343-H06C-GD62
GCATAGAGTTTTGATCTAACAAGAGACCATAAAGACTAGACAAAACCACTCACTCTGCAT
CACTATTAAGATATGATCTGAATCGAGGTCAAGTTTGCATTAAGTATCATATGGGAAATT
CTAATCAAACATGAGTAAGTAGTAGTAGTTGAAAGATACAAAACTTACCAGATTTCGCTG
ATCAGGGGATAGAGAAGTATCAGTAACAGTCCAACGCAAGCTTTTTGCAAGAATATCCTT
TTGAACTTTCCAACCCTTCTCTACATTGTAAATCCGAATACGGCTACCCTGAAAAAGAAG
GATTTGATTAATGCAAGGTATGTAGATTGATTCATCCAGAAAATCCTTCTTTTCTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT
   
Sequence Name 63-K033710-022-343-H06C-8409
GenBank Accession LN510919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K033710-022-343-H06C-8409
TTACAATTGAATATATCCTGAGAAAAGAAGGATTTTCTGGATGAATCAATCTACATACCT
TGCATTAATCAAATCCTTCTTTTTCAGGGTAGCCGTATTCGGATTTACAATGTAGAGAAG
GGTTGGAAAGTTCAAAAGGATATTCTTGCAAAAAGCTTGCGTTGGACTGTTACTGATACT
TCTCTATCCCCTGATCAGCGAAATCTGGTAAGTTTTGTATCTTTCAACTACTACTACTTA
CTCATGTTTGATTAGAATTTCCCATATGATACTTAATGCAAACTTGACCTCGATTCAGAT
CATATCTTAATAGTGATGCAGAGTGAGTGGTTTTGTCTAGTCTTTATGGTCTCTTGTTAG
ATCAAAACTCTATGCTTATATTCCAAAGAAAGGAATAATGAATTATTGCTACATTCAGGG
TTTACG

Last Updated on 06.12.2018 16:11