Sequences from confirmation amplicon


Confirmation sequences for line 321D07 and hit At4g04020 are shown.
Line ID 321D07
Locus At4g04020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1933184
Primers used for PCR 02yi - 8474, show primer details
   
Sequence Name 04-K122381-0022-321-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K122381-0022-321-D07C-8409
TTGAATATTTGCCATTTGTGGTTTTAGGGGATGATTCTTGATTTACCTGGACACGTTTAG
GACTTCGGATTTCAAACTTAGCGTTGGTGCTAAACGACGTTGTGGAAAACGGACCAGCGA
ACCGGACAGAGTTTTGAACGGTGAAGCTATCGGAATCAATGGTTTGTGAGATCTCATCCA
CTTTAACCAACGGTTCAATTCTTCGTGAGAGCAATGGGAACAACCCCACGAACGACGTGT
AGCTTCACGAGATAAACACTTAAAAACTAAGTTCTTGATTCATAACAATAACGGCTAAAA
ATAAACGAAGGCTCATAACTACACACAGAATCATAATCCAAATTCATAATTAAATCTTCT
TTGTAACCACAACTTGCTTTTCACTATCAAAATCAAATCATTCATAACGGAACTAAACAG
TTTTCACCGGGAAATTAAACTTCCACCGGTAAGTTTAAAGTATCATTAACTTAATTGGTA
AAGATCCTAGACGAAGCTTAGAGATCACCGGTTTGAGTAATGTTTGGGACAAAACTAATA
TTACATGCCGTGCCTAGGAGAGTATCAGAACTAAAAGTACTTACGCGAGGATCCATTTGC
CGTTGAGGAGAAACAGAGCTTCGTTAGGAGCTGGAGTAGGGTTCTTAGACTCGAGCTGTG
TGATGAGCTCGCTGATCTCAGCTCTCGTATCACTCGATACGCTTAAACCTCGATCTGTTC
CGTACAACGAATCCGCTAGTGATCTCTTTTAGCCT
   
Sequence Name 28-K122382-0022-321-D07C-02yi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02yi: 5'-AGAGGCTAAAGAGATCACTAGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K122382-0022-321-D07C-02yi
GAGAGCTGAGATCAGCGAGCTCATCACACAGCTCGAGTCTAAGAACCCTACTCCAGCTCC
TAACGAAGCTCTGTTTCTCCTCAACGGCAAATGGATCCTCGCGTAAGTACTTTTAGTTCT
GATACTCTCCTAGGCACGGCATGTAATATTAGTTTTGTCCCAAACATTACTCAAACCGGT
GATCTCTAAGCTTCGTCTAGGATCTTTACCAATTAAGTTAATGATACTTTAAACTTACCG
GTGGAAGTTTAATTTCCCGGTGAAAACTGTTTAGTTCCGTTATGAATGATTTGATTTTGA
TAGTGAAAAGCAAGTTGTGGTTACAAAGAAGATTTAATTATGAATTTGGATTATGATTCT
GTGTGTAGTTATGAGCCTTCGTTTATTTTTAGCCGTTATTGTTATGAATCAAGAACTTAG
TTTTTAAGTGTTTATCTCGTGAAGCTACACGTCGTTCGTGGGGTTGTTCCCATTGCTCTC
ACGAAGAATTGAACCGTTGGTTAAAGTGGATGAGATCTCACAAACCATTGATTCCGATAG
CTTCACCGTTCAAAACTCTGTCCGGTTCGCTGGTCCGTTTTCCACAACGTCGTTTAGCAC
CAACGCTAAGTTTGAAATCCGAAGTCCTAAACGTGTCCAGGTAAATCAAGAATCATCCCC
TAAAACCACAAATGGCAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAA

Last Updated on 06.12.2018 16:11