Sequences from confirmation amplicon


Confirmation sequences for line 107G03 and hit At4g04570 are shown.
Line ID 107G03
Locus At4g04570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2292558
Primers used for PCR CD48 - 8474, show primer details
   
Sequence Name 27-K104189-0022-107-G03C-CD48
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CD48: 5'-AGAAGGACTTGCAGCTTTTGTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104189-0022-107-G03C-CD48
TTTTTTTTTTTTAAACCTGGAGGTTTGGGGCCAAACCCCGTAACCCCCCCGGGACCAAAC
CCCCACCAGGAAAAATTATTTTCTTCCCAAGCGTTACTCAAACCGGCAACCTTAACCTTC
TCCACATATCATTTTTGGTTTTTCTACCTCCAAAACTTTACCCCAAAAGAAAAAACTTAT
TTTTTTGGGGTAGGCAGGAAAAAAAGGGGTTAAAGGAAACCCGGAAATTAAAATTGACCC
TTTCTTAAAAAAAAACCCAAAAAAGGAAACCACCAATTTAACCCAAATGGGTTTGTTGGG
GGTTCAAAAAAATTCAACCCACCAAGGGATCCATTTGAAAAGGGCTCCAGGCCCGGAAAA
TCTCCTGGGATCACCAAGGAGTATGAGGGCCAATAGGGAAAAAAAAAAAAAGTATTTCCC
ATTTTTTTTTCATTTCAAAAATGTAAATTCCCCCCCCGTTATAAAAAAA
   
Sequence Name 43-K104190-0022-107-G03C-8409
GenBank Accession LN504821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K104190-0022-107-G03C-8409
TTTACATTGAATCATTGTTGTGTTGAATTCTCTTGAACACACAACAAACCAATTTGGATC
AACTTGATGATCTCATTTCTTGGATTCTCTATTAAGAACGGATCAATTATAATCTCAGGC
TTTCCTTCAACCCATCTTTTCCATGCCTACACCAAAAACATAAGTTCTTACTTTCGGGAT
AAAGTTTTGAAGATAGAAAAACAAAAACTGATATGTGAAGAAGCTTAAGGTCGCCGGTTC
GAGTAACGCTTGGAACGAAACTAATATTACATGCTGTGGTTTCGGTCCTGGGGGGATTAC
GGGCTTCGGCCCAAAACCTCCAGCTATTCAAAAAAAAAAAAATTTGTAAAAAACCTTAAA
AAACCTGC
Primers used for PCR CD49 - 3144, show primer details
   
Sequence Name 40-K104270-0022-107-G03C-CD49
GenBank Accession LN503696 [GenBank]
Sequencing primer gene specific primer, CD49: 5'-CGAATCCAAGATTATGCGATTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104270-0022-107-G03C-CD49
TATTCAATATTTTGATTAACTGATGTATATCTCAACGACAACTCAACTCCGTGAAGACAT
CGTCGCTCAATGACATTGCACCACTTTCAGATTGGGATCGAATCCAAGTGAAAGCAGGAG
CTTTAGGTAAAGGAATGATGATAGTCTCACTGCCAAGCCAAATTATTACAGAGCTCATGG
ACACGATGCTCAGGAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 56-K104271-0022-107-G03C-3144
GenBank Accession LN509091 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K104271-0022-107-G03C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTCCTGAGCATCGTGTCCATGAGCTCTGTAATAAT
TTGGCTTGGCAGTGAGACTATCATCATTCCTTTACCTAAAGCTCCTGCTTTCACTTGGAT
TCGATCCCAATCTGAAAGTGGTGCAATGTCATTGAGCGACGATGTCTTCACGGAGTTGAG
TTGTCGTTGAGATATACATCAGTTAATCAAAATATTTGAATATTTTTTAATATCAAACTC
CATTAATCTGCAAATTTGTTAGTAAATCGCANAATCTTGGATTC

Last Updated on 06.12.2018 16:11