Sequences from confirmation amplicon


Confirmation sequences for line 546D04 and hit At4g04620 are shown.
Line ID 546D04
Locus At4g04620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2330235
Primers used for PCR KG25 - 8474, show primer details
   
Sequence Name 63-K038030-022-546-D04C-KG25
GenBank Accession LN510930 [GenBank]
Sequencing primer gene specific primer, KG25: 5'-GTTATCCATTCGGTTTCAGTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K038030-022-546-D04C-KG25
TTTTTCGGTTCTAAGTTTAGTATTCATTTGGTTATTTTGAAAGTTTGATTCGTATCGGTT
TTTTCAGTTTTAAAATTTAATATCCATTCTATTATTTTGCTTTGAATATTTTTGGATATT
TCGGTTATTTTGGATATTTTCGATCATTTTTGAATATTTTCGGTTATTTTTTCGAGTATT
TTTACTATTTTATATATCTTTTCAATTATTTTTAATTATGTAGTCTAAAAATAAATAATA
TTTAGTATCCATTTAGTTTTGGTTCTTTCGGTTCTTTCATTTATAGAATTTAGTATCCAT
TCGAATATTTTCCAATTTTTGGTTTGCTTTGATTTAATTAGTTTTGCTTTGATTTTGATT
CGGTATATTCGAGTTGGTTTATTTGTCCACGCCTAGTAGTCCTGATCATGTAAATGACAG
AAAAGCCCAAACGAGATCCAAACCTATAAAGGCCCAATAAATAGTTCACAAACTCATGAC
CACTACAATCCAACGACGTCGTATCATCTCGCTGACGTAAGATAAGCATCGTAACTAATT
AAAATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACAT
   
Sequence Name 07-K038030-022-546-D04C-8409
GenBank Accession LN489185 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K038030-022-546-D04C-8409
CATTTCAATTGAATATATCCTGGATTTTAATTAGTTACGATGCTTATCTTACGTCAGCGA
GATGATACGACGTCGTTGGATTGTAGTGGTCATGAGTTTGTGAACTATTTATTGGGCCTT
TATAGGTTTGGATCTCGTTTGGGCTTTTCTGTCATTTACATGATCAGGACTACTAGGCGT
GGACAAATAAACCAACTCGAATATACCGAATCAAAATCAAAGCAAAACTAATTAAATCAA
AGCAAACCAAAAATTGGAAAATATTCGAATGGATACTAAATTCTATAAATGAAAGAACCG
AAAGAACCAAAACTAAATGGATACTAAATATTATTTATTTTTAGACTACATAATTAAAAA
TAATTGAAAAGATATATAAAATAGTAAAAATACTCGAAAAAATAACCGAAAATATTCAAA
AATGATCGAAAATATCCAAAATAACCGAAATATCCAAAAATATTCAAAGCAAAATAATAG
AATGGATATTAAATTTTAAAACTGAAAAAACCGATACGAATCAAACTTTCAAAATAACCA
AATGAATACTAAACTTTAGAACCGAAAAAGTTGAAACCGAATAGATACAAACTGA

Last Updated on 06.12.2018 16:11