Sequences from confirmation amplicon


Confirmation sequences for line 819C04 and hit At4g05140 are shown.
Line ID 819C04
Locus At4g05140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2651858
Primers used for PCR DA35 - 8474, show primer details
   
Sequence Name 39-K107369-0022-819-C04C-DA35
GenBank Accession LN503375 [GenBank]
Sequencing primer gene specific primer, DA35: 5'-GGAGCAAGTGAGTTTAACGTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K107369-0022-819-C04C-DA35
AAGCTATTAGTGTTGAAAACTTCATGTTTTGCTTACATTTTATTTATTCATGCAGTGATC
TTTTTAGCTATCTCCACATTGGTAGAGTTTCTATGTGTTCTGCTATACGCATATGTGTTC
CCGAAACTTCCAATTGTTAAATACTACCGAAGTAAAGCTGCTTCTGAAGGATCCAAAACT
GTTTATGCTGATCTTGCTGCTGCTGGTATCCAAAACCAATCAGTTTTGGTAAGTTTAAAT
CCCTCAAGATCTGTCCCTTTGTGGTTATTTTAAAATATTCTTTTAAAAACCTTCACCATT
TTCTATTTCTTCAGATTCTATTCATTTTTAAAGACTCCTTCATTCTTAAGTCTTAAACAT
TGTTTGCTTCTTGATTATATTCTTCTTTGTGGTTATTGAATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 55-K107370-0022-819-C04C-8409
GenBank Accession LN508832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107370-0022-819-C04C-8409
GCCATTTACATTGAATATATTCATAACCACAAAGAAGAATATAATCAAGAAGCAAACAAT
GTTTAAGACTTAAGAATGAAGGAGTCTTTAAAAATGAATAGAATCTGAAGAAATAGAAAA
TGGTGAAGGTTTTTAAAAGAATATTTTAAAATAACCACAAAGGGACAGATCTTGAGGGAT
TTAAACTTACCAAAACTGATTGGTTTTGGATACCAGCAGCAGCAAGATCAGCATAAACAG
TTTTGGATCCTTCAGAAGCAGCTTTACTTCGGTAGTATTTAACAATTGGAAGTTTCGGGA
ACACATATGCGTATAGCAGAACACATAGAAACTCTACCAATGTGGAGATAGCTAAAAAGA
TCACTGCATGAATAAATAAAATGTAAGCAAAACATGAAGTTTTCAACACTAATTAGCTTT
ATAGTTGTCAGATTTGAGATCTTAAGAAAACGTTAAACTCCCTTGCTC

Last Updated on 06.12.2018 16:11