Sequences from confirmation amplicon


Confirmation sequences for line 057C02 and hit At4g05230 are shown.
Line ID 057C02
Locus At4g05230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2687843
Primers used for PCR PC32 - 8474, show primer details
   
Sequence Name 11-K118356-0022-057-C02C-PC32
GenBank Accession LN491325 [GenBank]
Sequencing primer gene specific primer, PC32: 5'-TCTCGTATCCAACTTTCCATCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118356-0022-057-C02C-PC32
TCAGTGTTCAAACCGAGCAATTCCCCANTCAAATTCAACAGAACAGACCAGTAACGGTCA
TCATGAATCAACAGTGATGATCCCAATGAGCAACAACAACAACAACAACAACCCGAAGAA
GCTGAGAGTAATGGTGTTGCCGAAATCCGGGACTAGGAAGATTCCGGTGGACGTGAACGC
AGGGGATAATGTTGGAGAGCTGAGGAAAGAGTTGGCTAAGATTCAACAGAGGTTTCAGTT
AAGTCTGCCTCAAGAAGGCTATTTCTTTATCTATAAACAGAATGTAATGGATGAAAACAG
GTCATTCCGGTGGCACCGTGTTGATCAAGGCGACACAATTGAGATTTTCAACGGAAGTGT
TTCCGGTGGCTCTTAATACCCTATAATTTCGAAGCTCAAGTCTTTGCAATCTCCTCATTT
CTTGTAGCTTACAGTCTTTTTTTTTTTAAGTTTCTGGCTTTCTTCTAAATTGTATTTTCG
TCTCAAAGCTTTCTACAAATATGCTTAAAAAAATCTATGACAAGTAATTTTAGTATTTTA
CCAAAAAATAATAACGAGCTGAAAAAAAAAAACTTGTGTGTTATTGACTTACCTCTGAGT
ATGATGGTCAATATGAATAATTTGAATAATTTGATAATTTAGATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCATTTCAAAAATG
TAGATGTCC
   
Sequence Name 11-K118355-0022-057-C02C-8409
GenBank Accession LN491324 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118355-0022-057-C02C-8409
TTGACCATCATACTCAGAGGTAAGTCAATAACACACAAGTTTCTCTTTTTCAGCTCGTTA
TTATTTTTTGGTAAAATACTAAAATTACTTGTCATAGATCTTCTTAAGCATATTTGTAGA
AAGCTCTGAGACGAAAATACAATTTAGAAGAAAGCAAGAAACTTAAAAAAAAAAAGACTG
TAAGCTACAAGAAATGAGGAGATTGCAAAGACTTGAGCTTCGAAATTATAGGGTATTAAG
AGCCACCGGAAACACTTCCGTTGAAAATCTCAATTGGGTCGCCTTGATCAACACGGGGCC
ACCGGAATGACCTGTTTTCATCCATTACATTCTGTTTATAGATAAAGAAATAGCCTTCTT
GAGGCAAACTTAACTGAAACCTCTGTTGAATCTTAGCCAACTCTTTCCTCAGCTCTCCAA
CATTATCCCCTGCGTTCACGTCCACCGGAATCTTCCTAGTCCCGGATTTCGGCAACACCA
TTACTCTAAGCTTCTTCGGGTTGTTGTTGTTGTTGTTGTTGCTCATGGGGATCATCACTG
TTGATTCAGGATGACCGTTACGGGTCTGTTCTGTTGAATTTGACGGTGGGAATTGCTCGG
TTTTGAACACTTGAATGTTGTTACGGCTTTGGTCGGGAGAAAAGATGGA

Last Updated on 06.12.2018 16:11