Sequences from confirmation amplicon


Confirmation sequences for line 442B11 and hit At4g05540 are shown.
Line ID 442B11
Locus At4g05540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2820479
Primers used for PCR JH27 - 8474, show primer details
   
Sequence Name 57-K101270-0022-442-B11C-8409
GenBank Accession LN509361 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K101270-0022-442-B11C-8409
TTACAGCCGTGTTTGTAGGAGTGCACACAACTGTTTTGCAACTTAGGCTGAGAAGAGCAA
ACAGAAGAGTAGCGACTGTCTTTGTGTTACCGGTACCAGGGGGTCCCCATATCAGTTTCA
CACTAGTCTTGTCGCGGATTTCTCTTGTCTCAAGGCAAGCCAAAATTGCAGATTCTTGAG
AAGAGTTCAAATTCGCTGATCGTATAATATCTAACACATGATTCCCCCAATTCCTAGAAG
AAACAGATTGCTCTGTATCCTGAAATATTTACAAGAAAAACCACAGTTACTCCACATTAG
ACGCTAAAGCCAGATCATTTCATGTTTACTTACCGCGGTATTTGCTTGAAGGACACTCTT
GATGAGACTCAAGTTTCCATCTCCGTTGTGCAAAGCCTTCCAGATACGAGTGTTTGTGGT
TAAGTATATAAGAAAAAAACCCGAACGAAACGAATATTGCTCATCAATGGATATAGATTT
AGAGGAATGAACAGAGAAATGTAGCTCATCTCCAACAGAGAACACATACGCAAGAATCAG
TGGGTTTAAGTCATCC
   
Sequence Name 57-K101276-0022-442-B11C-JH27
GenBank Accession LN509362 [GenBank]
Sequencing primer gene specific primer, JH27: 5'-GTGGATGACTTAAACCCACTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K101276-0022-442-B11C-JH27
TGACTACATTTCTCTGTTCTTCCTCTAATCTATATCCATTGATGAGCAATATTCGTTTCG
TTCGGGTTTTTTTCTTATATACTTAACCACAAACACTCGTATCTGGAAGGCTTTGCACAA
CGGAGATGGAAACTTGAGTCTCATCAAGAGTGTCCTTCAAGCAAATACCGCGGTAAGTAA
ACATGAAATGATCTGGCTTTAGCGTCTAATGTGGAGTAACTGTGGTTTTTCTTGTAAATA
TTTCAGGATACAGAGCAATCTGTTTCTTCTAGGAATTGGGGGAATCATGTGTTAGATATT
ATACGATCAGCGAATTTGAACTCTTCTCAAGAATCTGCAATTTTGGCTTGCCTTGAGACA
AGAGAAATCCGCGACAAGACTAGTGTGAAACTGATATGGGGACCCCCTGGTACCGGTAAC
ACAAAGACAGTCGCTACTCTTCTGTTTGCTCTTCTCAGCCTAAGTTGCAAAACAGTTGTG
TGCACTCCTACAAACACGGCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11