Sequences from confirmation amplicon


Confirmation sequences for line 219G07 and hit At4g08320 are shown.
Line ID 219G07
Locus At4g08320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5253590
Primers used for PCR AC24 - 8474, show primer details
   
Sequence Name 09-K027788-022-219-G07C-8409
GenBank Accession LN490034 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K027788-022-219-G07C-8409
CATTTACATTGAAGCCTTTACAAAACTTATAACCAAACTCATAACCAACAGAAGAAGTAA
AACCGTCAAAGAAAATCAAACCTACATTGACACAATCATGAAATATCCTTGTAGCTTTCT
CCAGCTGAGCAGGATCATCATCTCCATCAGGTGTATTCCTAAAATACCGTACTTTCTCAA
GCGCACCAAAGAGTTGACCAAACAGTTCATCTCTAGAAGTCCCTAAAACCAGCACAATCC
CACAATAATAAGTTCCATTACTCTATCCACCACTCACATTGGTCCAATAAACACGAAACA
AACCATTACTTACCAGTAAAACTAGGCTCTTTACACGTATCCACGGAGACATGAGAACCG
GAGGAACTAGGATCCTGAGTAGTAGCAGCAACAGGTGGTGGTGGTGGTGGTGGGTATGGT
TTCAT
   
Sequence Name 10-K027788-022-219-G07C-AC24
GenBank Accession LN490508 [GenBank]
Sequencing primer gene specific primer, AC24: 5'-CATTGGACAACAATGAAACCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K027788-022-219-G07C-AC24
TACTCAGGATCCTAGTTCCTCCGGTTCTCATGTCTCCGTGGATACGTGTAAAGAGCCTAA
GTTTTACTGGTAAGTAATGGTTTGTTTCGTGTTTATTGGACCAATGTGAGTGGTGGATAG
AGTAATGGAACTTATTATTGTGGGATTGTGCTGGTTTTAGGGACTTCTAGAGATGAACTG
TTTGGTCAACTCTTTGGTGCGCTTGAGAAAGTACGGTATTTTAGGAATACACCTGATGGA
GATGATGATCCTGCTCAGCTGGAGAAAGCTACAAGGATATTTCATGATTGTGTCAATGTA
GGTTTGATTTTCTTTGACGGTTTTACTTCTTCTGTTGGTTATGAGTTTGGTTATAAGTTT
TGTAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACTT

Last Updated on 06.12.2018 16:11