Sequences from confirmation amplicon


Confirmation sequences for line 835C01 and hit At4g08810 are shown.
Line ID 835C01
Locus At4g08810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5616231
Primers used for PCR FV90 - 8474, show primer details
   
Sequence Name 45-K118874-0022-835-C01C-FV90
GenBank Accession LN505697 [GenBank]
Sequencing primer gene specific primer, FV90: 5'-TCTTTTCGCGTACTACGTTTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118874-0022-835-C01C-FV90
GATGATGTAGAATCACTTGGTTATATCCTCATGTACTCCCTCAAAGGAAGGTGAAAATCA
TGGAGTAACCTCTCTTTATATCCTTCTTCTTGTCTATCATTCCATCTCTTATCCTATTTT
ATTGTAGTCTCCCATGGAAGGGACTAAAAGCTGGGAATAAGAAACAGAAATATGACAAGA
TTAGCGAGAAGAAGGTTTCAACTTCCATCGAAGTAAGTTCTTTGTATATGGTGACACATT
TAGAAACTTCTATGAGTCTATGTACTTAACTTTGCATCATTGTCTTCGTAGACGTTATGT
GAAGGTCATCCAATAGAGTTTGCAACCTACATCCATTACTGCCGCTCGCTTAGGTTCGAT
GATAAGCCAGACTATGCCTATTTGAAGAGACTGTTCCGCGACCTTTTTATTCGTGAAGGT
GATTCATTTTCGATATCTCCTTGTTCCTCTCAGGATATATTAAGTTAATACTTCTGTTGC
CACCAATGGAGTATTTGTTTATCTCAGGTTTTCAGTTTGATTTTGTGTTTGACTGGACGG
TGTTAAAGCTGCCCGCAATCACAATGCCGAAACCCTCCACCTTGTGCAAATGCACACTCT
TGATACTTTCAACTTTTTGATCCCGTTCATGATACACAGATGAACAATACAAGATTGTTT
TGATCAATAAAAGAGTTCTAGTTAAAAGCATAATCATAAACAATATATTCCTGAATCGAT
ATGTATCAGCTATGTCACTTTGGTGTTGCCTTCTTACAGAATTAAGGATATGAATCAAAA
AATGTAAACGTTACGTACACTCTTTCATAGCTAGTAGAAACACAAGGTAAACACACAAGC
ATAACTGAATTTGATACACAAACAAATAAAACAATGAAAACATATCACAACCTGAATGAT
CATTTTAGCATGTATTGAT
   
Sequence Name 05-K118873-0022-835-C01C-8409
GenBank Accession LN488571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118873-0022-835-C01C-8409
TTGAATATATCCTGCAAAGACGGGATCAATACATGCTAAATTGATCATTCAGGTTGTGAT
ATGTTTTCATTGTTTTATTTGTTTGTGTATCAAATTCAGTTATGCTTGTGTTGTTTACCT
TGTGTTCTACTAGCTATTGAAGAGTGTACGTAACGTTTACATTTTTTGATTCATATCCTT
AATTCTGTAAGAAGGCAACACCAAAGTGACATAGCTGATACATATCGATTCAGGAATATA
TTGTTTATGATTATGCTTTTAACTAGAACTCTTTTATCGATCAAAACAATCTTGTATTGT
TCATCTGTGTATCATGAACGGGATCAAAAAGTTGAAAGTATCAAGAGTGTGCATTTGCAC
AAGGTGGAGGGTTTCGGCATTGTGATTGCGGGCAGCTTTAACACCGTCCAGTCAAACACA
AAATCAAACTGAAAACCTGAGATAAACAAATACTCCATTGGTGGCAACAGAAGTATTAAC
TTAATATATCCTGAGAGGAACAAGGAGATATCGAAAATGAATCACCTTCACGAATAAAAA
GGTCGCGGAACAGTCTCTTCAAATAGGCATAGTCTGGCTTATCATCGAACCTAAGCGAGC
GGCAGTAATGGATGTAGGTTGCAAACTCTATTGGATGACCTTCACATAACGTCTACGAAG
ACAATGATGCANAGTTAAGTACATAGACTCATAGAAGTTTCTAAATGTGTCACCATATAC
AAAGAACTTACTTCGATGGAAGTTGAAACCTTCTTCTCGCTAATCTTGTCATATTTCTGT
TTCTTATTCCCAGCTTTTAGTCCCTTCCATGGGAGACTACAATAAAATAGGATAAGAGAT
GGAATGATAGACAAGAAGAAGGATATAANGAGAGGTTACTCCATGATTTTC

Last Updated on 06.12.2018 16:11