Sequences from confirmation amplicon


Confirmation sequences for line 154C09 and hit At4g09350 are shown.
Line ID 154C09
Locus At4g09350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5932251
Primers used for PCR 03jk - 8474, show primer details
   
Sequence Name 75-K113193-0022-154-C09C-03jk
GenBank Accession LN513273 [GenBank]
Sequencing primer gene specific primer, 03jk: 5'-GTTTGTTTTGCTCACTGGTAATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113193-0022-154-C09C-03jk
GCAATTTTTCGTCACTAAGGAGATTGGTTTTGTCCTCATCAACCGATTTGGCCGGGTCGG
ATCTTCCTCCGATCCAGCCTTCGTCCGGGCTTTCCCAGTGGATTCTTGTGTCTACTCCTG
GAGATGGTTTTGACGGTTTAGTCGGGCCTTGTGACGCGTATATACGCGTCACTCTTCTTG
CTGTTATCCTTCTAAATGCTTTCGTGTCATCCGTGAAATGCGCGCCGTTTTGGATTTTGG
GTAGGATAATGCATGAGGTTCGTGCATAGGTAGAAGTTGCATAAGCCATGGCTCTGTGTT
GAAGATGGTTTGTTCTTTTTTTTTCTGCCCTTCATGTTTTTGGTTTCTCTTACCGCAGAA
ACTTGTTCCAGAACCTCATGATATGTGTTAATTCTACATTATGTTTTTCTAATTTTTTGT
TGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGTTATT
   
Sequence Name 38-K113192-0022-154-C09C-8409
GenBank Accession LN503046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K113192-0022-154-C09C-8409
TTGAATATCACAAAAAATTAGAAAAACATAATGTAGAATTAACACATATCATGAGGTTCT
GGAACAAGTTTCTGCGGTAAGAGAAACCAAAAACATGAAGGGCAGAAAAAAAAAGAACAA
ACCATCTTCAACACAGAGCCATGGCTTATGCAACTTCTACCTATGCACGAACCTCATGCA
TTATCCTACCCAAAATCCAAAACGGCGCGCATTTCACGGATGACACGAAAGCATTTAGAA
GGATAACAGCAAGAAGAGTGACGCGTATATACGCGTCACAAGGCCCGACTAAACCGTCAA
AACCATCTCCAGGAGTAGACACAAGAATCCACTGGGAAAGCCCGGACGAAGGCTGGATCG
GAGGAAGATCCGACCCGGCCAAATCGGTTGATGAGGACAAAACCAATCTCCTTAGTGACG
AAAAATTTGCAGAACTAATCAAAGACTCTTTTGATTCTCATTACCAGTGAGCAAAACAAA

Last Updated on 06.12.2018 16:11