Sequences from confirmation amplicon


Confirmation sequences for line 496D04 and hit At4g09435 are shown.
Line ID 496D04
Locus At4g09435 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5973782
Primers used for PCR 20pq - 8474, show primer details
   
Sequence Name 34-K119832w-0022-496-D04C-20pq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 20pq: 5'-TCCACATATTTGAGTTTCTTGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119832w-0022-496-D04C-20pq
CTTGTTGGTATTCTCTCGAAATCATGGTTGCTAAGGTCTAAATATGCTAAACTGGTGTTA
TAGCATAATAGCTGATCTTGCAGTGACTGAAGATTCTTGCAGTTGTCAATGCAAAGCTCT
ATCAAACCCCTCGGTTGGTTGTCTAGACCAGGAAGAGTGAGGGTGGGAGTAAGTAGTGGC
AATGCTTTGAGCTGACGGCAGTTTCGAAGAGTAAGACATTCCAGCTTGGTCAGTTGTGCC
ATGGTTTTGGGTAGATGAACGAAATCATTACCGGTGAGGTCCATTTTCCTTAAGGACAAC
AAACAATCAATGTCTTGTGGGATTTCTCGGATGCTTAAGTTGATGAGCTTTAGCTCAGTC
AAACAAGGAAAGCTTGAAAAGCTCTGACAACTGAAAGGAGCTTCTCTCGCCCCGTAGTTG
AACCTCTCAATGACGAGTGTTTTGATAAGGTGCAAGGGTTGAATTGAACCTGTTTTCTGT
GCCATCGTAATTTTCAATTGATCAGGGATCTGTTGCTCGCAAGTAAAAGACAAACACAAA
CAAAGCGCTGTTAATATGATGGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATATCCGCAGCCGTTATTA
   
Sequence Name 10-K119831w-0022-496-D04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119831w-0022-496-D04C-8409
CATTTCATTGACCATCATATTAACAGCGCTTTGTTTGTGTTTGTCTTTTACTTGCGAGCA
ACAGATCCCTGATCAATTGAAAATTACGATGGCACAGAAAACAGGTTCAATTCAACCCTT
GCACCTTATCAAAACACTCGTCATTGAGAGGTTCAACTACGGGGCGAGAGAAGCTCCTTT
CAGTTGTCAGAGCTTTTCAAGCTTTCCTTGTTTGACTGAGCTAAAGCTCATCAACTTAAG
CATCCGAGAAATCCCACAAGACATTGATTGTTTGTTGTCCTTAAGGAAAATGGACCTCAC
CGGTAATGATTTCGTTCATCTACCCAAAACCATGGCACAACTGACCAAGCTGGAATGTCT
TACTCTTCGAAACTGCCGTCAGCTCAAAGCATTGCCACTACTTACTCCCACCCTCACTCT
TCCTGGTCTAGACAACCAACCGAGGGGTTTGATAGAGCTTTGCATTGACAACTGCAAGAA
TCTTCAGTCACTGCAAGATCAGCTATTATGCTATAACACCAGTTTAGCATATTTAGACCT
TAGCAACCATGATTTCGAGAGAATACCAACAAGCATTAGACACCTGTCCTCACTGAACAC
TCTCTGCCTCAAGAACTGCAAGAAACTCAAATATGTGGA

Last Updated on 06.12.2018 16:11