Sequences from confirmation amplicon


Confirmation sequences for line 107D12 and hit At4g09830 are shown.
Line ID 107D12
Locus At4g09830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6189196
Primers used for PCR 03qt - 8474, show primer details
   
Sequence Name 88-K115779-0022-107-D12C-03qt
GenBank Accession LN514892 [GenBank]
Sequencing primer gene specific primer, 03qt: 5'-AACGAAAACGGAAGAGATAGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K115779-0022-107-D12C-03qt
ATCAGACCAGTAATATAGACGAAGAGACAAGGATAACGCAAGGTGAAAGTTAGAAACACG
GTCTCCAGAGAGACTTTTCTTGTTTCATTTCCATCTTCACGCTTCATAGCCTTGTTTTCC
TTTGTCATCAACTAGTTTTCATACTCTAAACTACAGTTCTAAAATGTCTAGACACATCAC
AATAAACAAAGGAAAGATAGACAGGAAGGATACTCAATGGCTGTGTTAGCAATCAAAGAC
ACTGGTTTAGGGGATGTGACTAAATCTTGTTGATTGAGAATGAAATGTGAGTAAAAGAGT
GTCTCTTTCAAACATATAGCTAAAGAACTGACTATAATTTGATTCAAGCTACATAAACAA
CTCAGTATATACTACCAATTCAAAAACCCTGAAAGACAGAAAGAAAGATGCATTCACATC
TTTGGCATAGGTCTCACTAATACAATTACACACAAACACGAGAAAACATAGACGTTTAGC
TACAAACACAATCTCTTGCTTCCAACAATAAGATAACAATTGCGGACAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGC
ACGGTTATT
   
Sequence Name 40-K115778-0022-107-D12C-8409
GenBank Accession LN503820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115778-0022-107-D12C-8409
ATCGGAATGAGCATTTACATTGAATATATCCTGTCCGCAATTGTTATCTTATTGTTGGAA
GCAAGAGATTGTGTTTGTAGCTAAACGTCTATGTTTTCTCGTGTTTGTGTGTAATTGTAT
TAGTGAGACCTATGCCAAAGATGTGAATGCATCTTTCTTTCTGTCTTTCAGGGTTTTTGA
ATTGGTAGTATATACTGAGTTGTTTATGTAGCTTGAATCAAATTATAGTCAGTTCTTTAG
CTATATGTTTGAAAGAGACACTCTTTTACTCACATTTCATTCTCAATCAACAAGATTTAG
TCACATCCCCTAAACCAGTGTCTTTGATTGCTAACACAGCCATTGAGTATCCTTCCTGTC
TATCTTTCCTTTGTTTATTGTGATGTGTCTAGACATTTTAGAACTGTAGTTTAGAGTATG
AAAACTAGTTGATGACAAAGGAAAACAAGGCTATGAAGCGTGAAGATGGAAATGAAACAA
GAAAAGTCTCTCTGGAGACCGTGTTTCTAACTTTCACCTTGCGTTATCCTTGTCTCTTCG
TCTATATTACTGGTCTGATTATAAACAGAACGAATGTTAGAACATCCGATCGTTGTCTAT
CTCTTCCGTTTTCGT

Last Updated on 06.12.2018 16:11