Sequences from confirmation amplicon


Confirmation sequences for line 501D10 and hit At4g09830 are shown.
Line ID 501D10
Locus At4g09830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6189818
Primers used for PCR 15xw - 8474, show primer details
   
Sequence Name 47-K119622-0022-501-D10C-15xw
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 15xw: 5'-CGTATAAAAGTGCGAAGTACCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K119622-0022-501-D10C-15xw
CTTTCAAATCTCCAATCTTCTTCGGATTCGACGAATCCGTCATCGCCGACGGAATCGTGG
CCGTGTAATATTAACAGATGGGGAAGAAGAGAAAATCAACAGCCACAAGCCTCGATGAGG
TAGATCGGACTGTTTACGCTTCTTTTCGCACCGCCGCTAACTCTTTATCTCAGCTTTATA
CTCAGTCTATGAATCATCAGAAGTTATCTTTTCAAGCCGGTGAACGCCATGGTTTGGTAT
TTTCTTATTTACTCTCTGTGACTGTTTCAATTTCTTGATTCTTTGTGTGATTCATTGTCT
TGACAACTCTCTAATTGAAAAAGTTTTGATTTTTCTCATTTCTGTGTTAGTTTTGCGTTT
TAATTTTGATTGTCTTTCACAGGGCTTGAGACCTCTTAGACAATTGTTTGTTATCTTATT
GTTGGAAGCAAGAGATTGTGTTTGTAGCTAAACGTCTATGTTTTCTCGTGTTTGTGTGTA
ATTGTATTAGTGAGACCTATGCCAAAGATGTGAATGCATCTTTCTTTCTGTCTTTCAGGG
TTTTTGAATTGGTAGTATATACTGAGTTGTTTATGTAGCTTGAATCAAATTATAGTCAGT
TCTTTAGCTATATGTTTGAAAGAGACACTCTTTTACTCACATTTCATTCTCAATCAACAA
GATTTAGTCACATCCCCTAAACCAGTGTCTTTGATTGCTAACACAGCCATTGAGTATCCT
TCCTGTCTATCTTTCCTTTGTTTATTGTGATGTGTCTAGACATTTTAGAACTGTAGTTTA
GAGTATGAAAACTAGTTGATGACAAAGGAAAACAAGGCTATGAAGCGTGAAGATGGAAAT
GAAACAAGAAAAGTCTCTCTGGAGACCGTGTTTCNTACTTTCACCTTGCGTTATCCTTGT
CTNCTCGTCTATATTACTGGTCTGAATATAA
   
Sequence Name 23-K119620-0022-501-D10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K119620-0022-501-D10C-8409
CATCATGTGGCTAGAAGCACCTATACAGGAAATATTAGCATTATAGCTAACGAAAACGGA
AGAGATAGACAACGATCGGATGTTCTAACATTCGTTCTGTTTATAATCAGACCAGTAATA
TAGACGAAGAGACAAGGATAACGCAAGGTGAAAGTTAGAAACACGGTCTCCAGAGAGACT
TTTCTTGTTTCATTTCCATCTTCACGCTTCATAGCCTTGTTTTCCTTTGTCATCAACTAG
TTTTCATACTCTAAACTACAGTTCTAAAATGTCTAGACACATCACAATAAACAAAGGAAA
GATAGACAGGAAGGATACTCAATGGCTGTGTTAGCAATCAAAGACACTGGTTTAGGGGAT
GTGACTAAATCTTGTTGATTGAGAATGAAATGTGAGTAAAAGAGTGTCTCTTTCAAACAT
ATAGCTAAAGAACTGACTATAATTTGATTCAAGCTACATAAACAACTCAGTATATACTAC
CAATTCAAAAACCCTGAAAGACAGAAAGAAAGATGCATTCACATCTTTGGCATAGGTCTC
ACTAATACAATTACACACAAACACGAGAAAACATAGACGTTTAGCTACAAACACAATCTC
TTGCTTCCAACAATAAGATAACAAACAATTGTCTAAGAGGTCTCAAGCCCTGTGAAAGAC
AATCAAAATTAAAACGCANAACTAACACAGAAATGAGAAAAATCAAAACTTTTTCAATTA
GAGAGTTGTCAAGACAATGAATCACACAAAGAATCAAGAAATTGAAACAGTCACAGAGAG
TAAATAAGAAAATACCAAACCATGGCGTTCACCGGCTTGAAAAGATAACTTCTGATGATT
CATAGACTGAGTATAAAGCTGAGATAAAGAGTTAGCGGCGGTGCGAAAAGAAGCGTAAAC
AGTCCGATCTACCTCATCGAGGCTTGTGGCTNGTGATTTTCTCTTCTTCCCATCTG

Last Updated on 06.12.2018 16:11