Sequences from confirmation amplicon


Confirmation sequences for line 526B02 and hit At4g10010 are shown.
Line ID 526B02
Locus At4g10010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6264513
Primers used for PCR CD73 - 8474, show primer details
   
Sequence Name 13-K106611-0022-526-B02C-8409
GenBank Accession LN492053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106611-0022-526-B02C-8409
TTACAATTGAATATATCCTGAATCACATTTACATTAACTTGTGTTTAGGAATTCACATGA
ATCATTTTGGTGCAATAATACTTGTATAAGGAGTTTTTATCACATACCTTCTTGCTTCTT
CATCACGGACCTTTGCATCAAGTTCCTTGCTTGGAGGATACTTTGGCAAACTTGATACAT
TACAAGGGAGTGGTTCCATAGTGAAGAACTAGAGAACAAAAGAAGAAGAAAGAATTGAGT
TTGCATTGAGGAGGTGAATCAGGAACTGCTAGTTGAAGAAATATAAAGCTACCTTGCTAC
TTAGTGTCGAAGAAGCCGTGCCACGCTTCGCTGGTTCTAAGGATAGAAGTTTGTCAACAA
GAGCTAGAGCAGAAGGTGGTAAGTTCTTGAAAGTCTCTAGAAGGACACGTTTATAAGGTT
GTTGCGGCTTGAAACTTGTTGCAAGTGGTAGCTTTGTCTTTTGCCAGTAATCATCACTT
   
Sequence Name 29-K106612-0022-526-B02C-CD73
GenBank Accession LN499303 [GenBank]
Sequencing primer gene specific primer, CD73: 5'-CAAGTGATGATTACTGGCAAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106612-0022-526-B02C-CD73
GTTCAGCCGCACAACCTTATAAACGTGTCCTTCTAGAGACTTTCAGAACTTACCACCTTC
TGCTCTAGCTCTTGTTGACAAACTTCTATCCTTAGAACCAGCGAAGCGTGGCACGGCTTC
TTCGACACTAAGTAGCAAGGTAGCTTTATATTTCTTCAACTAGCAGTTCCTGATTCACCT
CCTCAATGCAAACTCAATTCTTTCTTCTTCTTTTGTTCTCTAGTTCTTCACTATGGAACC
ACTCCCTTGTAATGTATCAAGTTTGCCAAAGTATCCTCCAAGCAAGGAACTTGATGCAAA
GGTCCGTGATGAAGAAGCAAGAAGGTATGTGATAAAAACTCCTTATACAAGTATTATTGC
ACCAAAATGATTCATGTGAATTCCTAAACACAAGTTAATGTAAATGTGATTCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACGGTTTATT

Last Updated on 06.12.2018 16:11