Sequences from confirmation amplicon


Confirmation sequences for line 770D01 and hit At4g11070 are shown.
Line ID 770D01
Locus At4g11070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6760734
Primers used for PCR IS66 - 8474, show primer details
   
Sequence Name 05-K025042-022-770-D01C-8409
GenBank Accession LN488195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K025042-022-770-D01C-8409
GATTTCCCCNACTTTTTTCCACAATTCGATTTAAGTAGTACAGTATTGAATATTCTTGAG
ATTAATATAATCCCGATTAGCGCCTAGCGATTTAAGTAGCAGAGTCATTTTGCATAAGTA
GAGATGTTCAATATGAGCAAACTTGAACATTCTCTAATTTTTCTTTCTCTACGACGAAGT
CTTGGTCTTATTGTACTCTTCCACTTCTTTCTTGTAAGCTTCCATTAACTCAGCAGCTTT
GCTATTGTAAACTTGTTTCTCCTCTTCACCAAGTTCCTACAACAAAATTGTAACCAAAAC
CAACTCAATTTCACAACTAACAGTGATAAAATCTTACTCGTTGGATTCGAGTTTCATGAT
TTATAATATACCATCCATTTCAATGAAATGTGAGCAGTAACAGTTGAGTTGTTGATCCCA
GGATGTTCTTCTAACACACTCTTTCTTGCGTCTTTGCTGTAAAAAGATTTGAGATTATAA
AACCAGTTTAGCATCTGATTGTTTT
   
Sequence Name 06-K025042-022-770-D01C-IS66
GenBank Accession LN488645 [GenBank]
Sequencing primer gene specific primer, IS66: 5'-TTTAAAACCCTGAATCCACAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K025042-022-770-D01C-IS66
ATGCTAAACTGGTTTTATAATCTCAAATCTTTTTACAGCAAAGACGCAAGAAAGAGTGTG
TTAGAAGAACATCCTGGGATCAACAACTCAACTGTTACTGCTCACATTTCATTGAAATGG
ATGGTATATTATAAATCATGAAACTCGAATCCAACGAGTAAGATTTTATCACTGTTAGTT
GTGAAATTGAGTTGGTTTTGGTTACAATTTTGTTGTAGGAACTTGGTGAAGAGGAGAAAC
AAGTTTACAATAGCAAAGCTGCTGAGTTAATGGAAGCTTACAAGAAAGAAGTGGAAGAGT
ACAATAAGACCAAGACTTCGTCGTAGAGAAAGAAAAATTAGAGAATGTTCAAGTTTGCTC
ATATTGAACATCTCTACTTATGCAAAATGACTCTGCTACTTAAATCGCTAGGCGCTAATC
GGGATTATATTAATCTCAAGAATATTCAATACTGTACTACTTAAATCGAATTGTGGAAAA
AAGTGGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAATTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11