Sequences from confirmation amplicon


Confirmation sequences for line 533C08 and hit At4g11470 are shown.
Line ID 533C08
Locus At4g11470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6968995
Primers used for PCR CH04 - 8474, show primer details
   
Sequence Name 29-K106739-0022-533-C08C-CH04
GenBank Accession LN499306 [GenBank]
Sequencing primer gene specific primer, CH04: 5'-TCACATACTCTGGAGGCATGTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106739-0022-533-C08C-CH04
GGTTATGTAATCAGAAACAAACAAATAGAAAAAGGTTTATCAATTTTAAAGACATTATCG
GAGTACTAACAAGGTTCCAACAACTCTTCCTGTCTGGTCTTCAGTTTGGTCCACTCTAAA
ATTCCTTGCCATTCCAAAATCAGCTATTTTAGGGTTCATATCAGCATCTAAAAGTATGTT
ACTTGCTTTGATGTCACGGTGTATGATTGTTAGCCTTGAGTCTTGATGAAGATAGAGAAG
TCCTCGCGTAACCCCTCCTATGATGTTGTACCGTCTTTTCCAATCTAGTTGACTTTTCAT
TTTTGGATCTAAATTACACTATAATTATAAGAAAATTTGATGAATGAACATAAATTAAAC
CAAGTGTTATTGTTTATTACCAAAGAGGAAATAATCAAGGCTCTTGTTGGAAACAAACTC
GTAGACAAGTATCTGTTCATCTCTTTCTATGCAAAACCCAAGAAGCCTAACAAGATTCTT
GTGTTGAAGTAAGGCCGCAAACACAACCTCGTTCTTGAACTCATGTGTGCCTTGTCTTGA
ATTACTCGATAGACGCTTTACCTCAATATCTTGTGTTTGTCCAGGAAAGATAAAATTG
   
Sequence Name 13-K106738-0022-533-C08C-8409
GenBank Accession LN492056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106738-0022-533-C08C-8409
TTACATTGAATATATCCTGGACAAACACAAGATATTGCGGTAAACCTTCTATCGAGTAAT
TCAGGACAAGGCACACAAGAGTTCAACAACGAGGTTGTGATTGTGGCCAAACTTCAACAC
AAGAATCTTGTTAGGCTTCTTGGGTTTTGCATAGAAAGAGATGAACAGATACTTGTCTAC
GAGTTTGTTTCCAACAAGAGCCTTGATTATTTCCTCTTTGGTAATAAACAATAACACTTG
GTTTAATTTATGTTCATTCATCAAATTTTCTTATAATTATAGTGTAATTTAGATCCAAAA
ATGAAAAGTCAACTAGATTGGAAAAGACGGTACAACATCATAGGAGGGGTTACGCGAGGA
CTTCTCTATCTTCATCAAGACTCAAGGCTAACAATCATACACCGTGACATCAAAGCAAGT
AACATACTTTTAGATGCTGATATGAACCCTAAAATAGCTGATTTTGGAATGGCAAGGAAT
TTTAGAGTGGACCAAACTGAAGACCAGACAGGAAGAGTTGTTGGAACCTTGTTAGTACTC
CGATAATCTCTTTAAAATTGATAAACCTTTTTCTATTTGTTTGTTTCTGATTAACATAAA
CCTTTTTATGTTGTATATTTGCAGCGGTTACATGCCT

Last Updated on 06.12.2018 16:11