Sequences from confirmation amplicon


Confirmation sequences for line 090B02 and hit At4g11480 are shown.
Line ID 090B02
Locus At4g11480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6972681
Primers used for PCR 02fr - 8474, show primer details
   
Sequence Name 37-K111470-0022-090-B02C-02fr
GenBank Accession LN502657 [GenBank]
Sequencing primer gene specific primer, 02fr: 5'-GTACCGTCTTTTCCAATCTAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K111470-0022-090-B02C-02fr
TATTTTTAAAACTTTGGTAAATGCACATAATTAAACCAAGTAAGTGTTTTTGTTTATTAC
CAAAGAGGAAATAATTAAGGCTCTTGTTGGGAACAAACTCGTAGACAAGTATCTGTTCAT
CTCTTTCTAAGCAAAACCCAAGAAGCCTAACAAGATTCTTATGTTGAAGCTTAGCCACAA
TTACAACCTCGTTCTTGAACTCTTGTGTGCCTTGTCCTGAATTACTCTTGAACTCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGATTATTA
   
Sequence Name 21-K111469-0022-090-B02C-8409
GenBank Accession LN495719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K111469-0022-090-B02C-8409
TTCATTGAATTATCCTGAGTTCAGAGTAATTCAGGACAAGGCACACAAGAGTTCAAGAAC
GAGGTTGTAATTGTGGCTAAGCTTCAACATAAGAATCTTGTTAGGCTTCTTGGGTTTTGC
TTAGAAAGAGATGAACAGATACTTGTCTACGAGTTTGTTCCCAACAAGAGCCTTAATTAT
TTCCTCTTTGGTAATAAACAAAAACACTTACTTGGTTTAATTATGTGCATTTTACCAAAT
GTTTTTAAAAATATTTGTCTTATGCAGATCCAACCAAGAAGAGTCAGCTAGATTGGAAAA
GACGGTA

Last Updated on 06.12.2018 16:11