Sequences from confirmation amplicon


Confirmation sequences for line 659H03 and hit At4g13610 are shown.
Line ID 659H03
Locus At4g13610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7915842
Primers used for PCR MC80 - 8474, show primer details
   
Sequence Name 42-K102175-0022-659-H03C-8409
GenBank Accession LN504413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102175-0022-659-H03C-8409
GCCATTTACATAACTTAATGAGAGCAAGGTCTTGACATCTGATCAACTAATGGATGGTGG
TGAGGACGAGGACCTAAAATATGCTAAACTACTGCAAGAGGAAGAACATATGAAATCTAT
GGATCGGTCGAGAAATAAGAGAAGTTCAACAACTTCTGCTCCAAATAAGTTCTACATTAA
GATAAATGAAGATGAGATTGCCCATGATTATCCTCTCCCATCTTACTATAAAAACACCAA
AGATGAAACAGATGAACTTGTACTCTTTAATGCGGGCTATGCGGTTGATGCGAGGAACCT
ACCTTGCAGAACACTTCACAACTGGGCACTTTACAATTCTGATTTAATGTTGATATCTCT
TGAGTTTCTCCCGATGAAGCCATGTGCTGATATCGATGTCACCTATTTGGGTCAGGTGAG
GTGGCTCAAGATGATGGAAGGGTTTTTTTTCTCGATGATTCGGAGAACTCTACGCAATCA
CATGATCATGATGGAATAAACATATTCCTTACTCAGATAAAGGAATGGAAGATTGATTTT
GGGGAAGACATGATATTTGTTCTATTACGAACAGATATGG
   
Sequence Name 42-K102179-0022-659-H03C-MC80
GenBank Accession LN504414 [GenBank]
Sequencing primer gene specific primer, MC80: 5'-TAAGCTTACCAGGCCATATCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K102179-0022-659-H03C-MC80
TCTTCCCAAATAATCTTCCATTCCTTTATCTGAGTAAGGAATATGTTTATTCCATCATGA
TCATGTGATTGCGTAGAGTTCTCCGAATCATCGAGAAAAAAAACCCTTCCATCATCTTGA
GCCACCTCACCTGACCCAAATAGGTGACATCGATATCAGCACATGGCTTCATCGGGAGAA
ACTCAAGAGATATCAACATTAAATCAGAATTGTAAAGTGCCCAGTTGTGAAGTGTTCTGC
AAGGTAGGTTCCTCGCATCAACCGCATAGCCCGCATTAAAGAGTACAAGTTCATCTGTTT
CATCTTTGGTGTTTTTATAGTAAGATGGGAGAGGATAATCATGGGCAATCTCATCTTCAT
TTATCTTAATGTAGAACTTATTTGGAGCAGAAGTTGTTGAACTTCTCTTATTTCTCGACC
GATCCATAGATTTCATATGTTCTTCCTCTTGCAGTAGTTTAGCATATTTTAGGTCCTCGT
CCTCACCACCATCCATTAGTTGATCAGATGTCAAGACCTTGCTCTCATTAAGTTATGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11