Sequences from confirmation amplicon


Confirmation sequences for line 909G09 and hit At4g15910 are shown.
Line ID 909G09
Locus At4g15910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9029173
Primers used for PCR 36us - 8474, show primer details
   
Sequence Name 40-K117356-0022-909-G09C-36us
GenBank Accession LN503849 [GenBank]
Sequencing primer gene specific primer, 36us: 5'-TAAATATCATAAAAATGGCCGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117356-0022-909-G09C-36us
ATCTCTTTGCTCGCCGCATCCGGAGTCTGTCTTGTTCCATTGTCTTAAGGTACTTATGTT
TGAACTGATCCCATTTTTTCAGGTTACAATATATAAACATTTACACACTCTTTCATTTGC
TTAGATCGTATAAAGAATTTATAATTCTAATTGAATAATAGTTGATGGTTTGAATTTGAG
TGAAGGCTAAAGATTGATCTATCATTAATAAACGTAATTAATTTACTTAGATCTATTAAA
GATAATTATGCATGATATTATCACGTTATTTTCTACGTTTTCGTTATGTTATTTAGTCAT
TTGCTGTTTATATAATCTTACCTTCAAAATAAATTATTGGAATGTAGGAGGAGTTACGTT
GCTACATCGCAGAATGTAACAGCAGCAGGATTGAGTAAGGGAGGTTCCACCAGAGTTATG
GTGGGGAAGATGGAACAGAGAGGTTTAGATCAAGAGGCAGAGTCTGCTTGGGGACCAGAT
CCAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCA
   
Sequence Name 20-K117355-0022-909-G09C-8409
GenBank Accession LN495387 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117355-0022-909-G09C-8409
ACATTGGTATTGGATCTGGTCCCCAAGCAGACTCTGCCTCTTGATCTAAACCTCTCTGTT
CCATCTTCCCCACCATAACTCTGGTGGAACCTCCCTTACTCAATCCTGCTGCTGTTACAT
TCTGCGATGTAGCAACGTAACTCCTCCTACATTCCAATAATTTATTTTGAAGGTAAGATT
ATATAAACAGCAAATGACTAAATAACATAACGAAAACGTAGAAAATAACGTGATAATATC
ATGCATAATTATCTTTAATAGATCTAAGTAAATTAATTACGTTTATTAATGATAGATCAA
TCTTTAGCCTTCACTCAAATTCAAACCATCAACTATTATTCAATTAGAATTATAAATTCT
TTATACGATCTAAGCAAATGAAAGAGTGTGTAAATGTTTATATATTGTAACCTGAAAAAA
TGGGATCAGTTCAAACATAAGTACCTTAAGACAATGGAACAAGACAGACTTCCGGATGCG
GCGGAGCAAAGAGATTTAACGGCACCGGAGAGTGAACGAGCGGCCATTTT

Last Updated on 06.12.2018 16:11