Sequences from confirmation amplicon


Confirmation sequences for line 159D11 and hit At4g19510 are shown.
Line ID 159D11
Locus At4g19510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10635732
Primers used for PCR 05xt - 8474, show primer details
   
Sequence Name 41-K119622-0022-159-D11C-05xt
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 05xt: 5'-GATAATGATGAATGTGTCCGTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K119622-0022-159-D11C-05xt
GCAGACACGGATGCTGATATCATATATAACAGAACTTCCAGAGGTTTGATGGATACGAGT
TCCTTGAAGACATTGAAGATAACTCTAAGCGGTATGGATTACCTTATTTGTACCAAAAAC
TCCTCCATAAATTATTGGATGGAGAAAATGTAGATGTCCGCACCGTTGTAATACCGGAAA
ACTTTCTAAGGAACAAGAAATTGTTTATTGTGCTGGACAATGTGACCGAAGAGAAACAAA
TAGAATATCTTATCGGAAAGAAGAATGTGTACAGGCAAGGAAGTAGGATTGTTATAATAA
CAAGAGACAAGAAACTGCTGCAGAAAAACGCTGATGCTACATATGTGGTTCCCAGATTAA
ATGACAGGGAAGCTATGGAGTTATTCTGCCTTCAGGTATTTGGCAACCACTATCCCACGG
AAGAATTTGTGGATCTATCAAACGATTTTGTTTGTTATGCTAAAGGGCTTCCGTTAGCTT
TGAAGTTGTTAGGTAAGGGTCTATTAACCCATGATATAAACTACTGGAAGAAGAAATTGG
AGTTTTTACAGGTAAATCCAGACAAGGAGCTTCAGAAAGAGCTGAAATCGAGTTATAAAG
CACTTGATGATGATCAGAAGAGCGTATTTCTGGACATAGCATGTTTTTTCAGGTCAGAGA
AAGCAGATTTTGTTTCAAGCATTCTGAAATCAGACGACATTGATGCTAAAGATGTGATGA
GAGAACTTGAGGAGAAGTGCCTTGTAACCATTTCTTACGATAGGATTGAGATGCATGATC
TATTGCATGCAATGGGGAAGGAAATTGGAAAAGAAAAATCCATCAGAAAGGCAGGCGAAC
GTCGTAAGTTGTGGACCACAAAGATATTCGTGATATCCTGGAGCATAACACGGTACATCA
TATCTACAGTTGGGTTACTT
   
Sequence Name 17-K119620-0022-159-D11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K119620-0022-159-D11C-8409
TTGAATATATCCTGACACAATGAGAACTGTGGAATTTCAGGAATTTGAGTTTTGACAACA
TCGTGAAAGCAGCAGGAAAAAGCTTGATTCTTCTGACTTCAGACATGTTCAAGAAGATGC
CTCTAACACATTCAGTGCCCTGGTAGCAAGAGAAAAAGATTAAGGAACAGTGAAAGGTCG
ACATAAAAACAAAATACCAGAAGAAGTAAACCAACTGTAGATATGATGTACCGTGTTATG
CTCCAGGATATCACGAATATCTTTGTGGTTCCACAACCTACGACGTTCGCCTGCCTTTCT
GATGGATTTTTCTTTTCCAATTTCCTTCCCCATTGCATGCAATAGATCATGCATCTCAAT
CCTATCGTAAGAAATGGTTACAAGGCACTTCTCCTCAAGTTCTCTCATCACATCTTTAGC
ATCAATGTCGTCTGATTTCAGAATGCTTGAAACAAAATCTGCTTTCTCTGACCTGAAAAA
ACATGCTATGTCCAGAAATACGCTCTTCTGATCATCATCAAGTGCTTTATAACTCGATTT
CAGCTCTTTCTGAAGCTCCTTGTCTGGATTTACCTGTAAAAACTCCAATTTCTTCTTCCA
GTAGTTTATATCATGGGTTAATAGACCCTTACCTAACAACTTCAAAGCTAACGGAAGCCC
TTTAGCATAACAAACAAAATCGTTTGATAGATCCACAAATTCTTCCGTGGGATAGTGGTT
GCCAAATACCTGAAGGCAGAATAACTCCATAGCTTCCCTGTCATTTAATCTGGGAACCAC
ATATGTAGCATCAGCGTTTTTCTGCAGCAGTTTCTTGTCTCTTGTTATTATAACAATCCT
ACTTCCTTGCCTGTACACATTCTTCTTTCCGATAAGATATTCTATTTGTTTTCTCTCNGT
CACATTGTCCAGCACAATAAACAATTTCTTGTTCCCTTAGAAGTTTTCCGGTCTTCCCTG
CGCTCTGACATCAACATTTTCTCCATCAAATAATTTAT

Last Updated on 06.12.2018 16:11