Sequences from confirmation amplicon


Confirmation sequences for line 069E04 and hit At4g21323 are shown.
Line ID 069E04
Locus At4g21323 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11344189
Primers used for PCR TA03 - 8474, show primer details
   
Sequence Name 13-K016202-022-069-E04C-8409
GenBank Accession LN491817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K016202-022-069-E04C-8409
TAGATTTCCCGGACATGAAGCCATTTATATTTCCATGACCTGTTTTTCAGGGTCAGAGTC
TGTACACAGGACCAGAAATTAGCTTCACAGATGTGATTTGTACTGGTGATCATAGTAATG
TTGATCAAATTACAAAGGGTAAAGTTATAATGCACTTCTCGATGGGTCCCGTTCGTCCGC
TGACACCGGATGTTGTTCAGAAAAACGGTGGTATCGGTCTAATTTATGTGAGAAATCCGG
GTGATAGTAGAGTTGAATGTCCCGTAAACTTTCCTTGTATCTACTTGGATATGGAAGTTG
GATCCGAGCTATACACCTACATCCAAACTAGGAGGTTATTTATCTTTTACAAACTTTGCT
CGGTCAAATTAATATCTCATCTTCTTGCATATGAGAATCTGAATTGCTGTTTTCTTGCAG
CTCAATGAAAATTAAGATAAGCCCATACAAGACAATAATTGGCGAAAGTGTAGCAAGTAA
AGTCGCAAAGTCATCGGCAAGAGGGCCTAGTTCATTTTCACCTGCCATTCTTAAGGTTTT
GTTTCCTTCATCTCTTATTCCGGATCACTAAATCTATCATCTATGTTTTATTCTTCAACT
AACTTGTTTGTATTTTCTCTGTAATGCAGCCGGATATAGCAGCTCCGGGTTTGACCTTGC
TAACACCTAGAATACCAACAGA
   
Sequence Name 14-K016202-022-069-E04C-TA03
GenBank Accession LN492279 [GenBank]
Sequencing primer gene specific primer, TA03: 5'-TTAGGATGCGATATCTTGAGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K016202-022-069-E04C-ta03
CGATTCCTGCAATAACCGGAGTTGCCATTGATGTTCCTGAGTAAACGAATTCTCTGGTGT
CTTCGTCTGTTGGTATTCTAGGTGTTAGCAAGGTCAAACCCGGAGCTGCTATATCCGGCT
GCATTACAGAGAAAATACAAACAAGTTAGTTGAAGAATAAAACATAGATGATAGATTTAG
TGATCCGGAATAAGAGATGAAGGAAACAAAACCTTAAGAATGGCAGGTGAAAATGAACTA
GGCCCTCTTGCCGATGACTTTGCGACTTTACTTGCTACACTTTCGCCAATTATTGTCTTG
TATGGGCTTATCTTAATTTTCATTGAGCTGCAAGAAAACAGCAATTCAGATTCTCATATG
CAAGAAGATGAGATATTAATTTGACCGAGCAAAGTTTGTAAAAGATAAATAACCTCCTAG
TTTGGATGTAGGTGTATAGCTCGGATCCAACTTCCATATCCAAGTAGATACAAGGAAAGT
TTACGGGACATTCAACTCTACTATCACCCGGATTTCTCACATAAATTAGACCGATACCAC
CGTTTTTCTGAACAACATCCGGTGTCAGCGGACGAACGGGACCCATCGAGAAGTGCATTA
TAACTTTACCCTTTGTAATTTGATCAACATTACTATGATCACCAGTACAAATCACATCTG
TGAAGCTAATTTCTGGTCCTGTGTACAGACTCTGACCCTGAAAAACAGGTCATGGAAATA
TAAATGGCTTCATGTCCGGGAAATCTACATGNGATCAGCATGAGTATG

Last Updated on 06.12.2018 16:11