Sequences from confirmation amplicon


Confirmation sequences for line 251H07 and hit At4g21534 are shown.
Line ID 251H07
Locus At4g21534 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11456698
Primers used for PCR 19qv - 8474, show primer details
   
Sequence Name 55-K115869-0022-251-H07C-19qv
GenBank Accession LN508905 [GenBank]
Sequencing primer gene specific primer, 19qv: 5'-AACAAGAGATTGCTTCCTCTAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K115869-0022-251-H07C-19qv
TCTAGATTCCTTTAGTTTGAATCAGGTGAGAATAGCACGAAAGACACACATCATCACTTC
CAATTTAAGTTATCCAAAAACAATTGACATAGAGAAACTGTATACTTCTTACATACCTCA
ACAAGGATACCGTCGCCGCTGACACAAACAATACCATCATATTTTGACACATCCATGGAC
TTAACAAATTCCTTTGCATGCAACTGATACTTAGTTTCTGAAGACTCAACCATAAGTAAT
CCATTGTTAATTTCCCAAAAAAAGAGTTTTTGAGACCCCAGAAAGAAACAAAAATAGAAA
CCAAGGAACCTTGAATCTCAAGTTGAACATCAGCATCTTCAAACAAAGGCTTCACTTCCT
TAACAAAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACGATTATTATA
   
Sequence Name 14-K115867-0022-251-H07C-8409
GenBank Accession LN492651 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115867-0022-251-H07C-8409
ATTTTTGTTAGGAAGTGAAGCCTTTGTTTGAGATGCTGATGTTCAACTTGAGATTCAAGG
TTCCTTGGTTTCTATTTTTGTTTCTTTCTGGGGTCTCAAAAACTCTTTTTTTGGGAAATT
AACAATGGATTACTTATGGTTGAGTCTTCAGAAACTAAGTATCAGTTGCATGCAAAGGAA
TTTGTTAAGTCCATGGATGTGTCAAAATATGATGGTATTGTTTGTGTCAGCGGCGACGGT
ATCCTTGTTGAGGTATGTAAGAAGTATACAGTTTCTCTATGTCAATTGTTTTTGGATAAC
TTAAATTGGAAGTGATGATGTGTGTCTTTCGTGCTATTCTCACCTGATTCAAACTAAAGG
AATCTAGATTTCATGTCTAAAACGAAGTTAGAGCTTTTGAAGTATCTGCTAGAGGAAGCA
ATCTCTTGTTA

Last Updated on 06.12.2018 16:11