Sequences from confirmation amplicon


Confirmation sequences for line 061E11 and hit At4g23160 are shown.
Line ID 061E11
Locus At4g23160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12133078
Primers used for PCR 05cs - 8474, show primer details
   
Sequence Name 51-K108895-0022-061-E11C-05cs
GenBank Accession LN507528 [GenBank]
Sequencing primer gene specific primer, 05cs: 5'-ACCAACTATTCGGCTTGTGTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K108895-0022-061-E11C-05cs
ATTCCAAATCTGCAATTTTCGGATTTATATCCGCATCTAGGAGAATGTTACTTGCTTTAA
GGTCACGGTGTATGATTGTGAGCCGTGAATCTTGATGAAGATATAGAATCCCCCGAGCAA
TTCCTCCAATGATATTGTATCGCTGCATCCAGTCCAGCTGAGTTTGCTTTGTAGGGTCTG
TGCAAATCCATGGATTACAGTTAAATCTTGAGATTAGCAACCCCAAGTTTCAAGATTAAT
GGTCAAAGAATCATTGAAATGCAACAGACCAAATAGTAAGCAATCAAGGCTTTTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TA
   
Sequence Name 35-K108894-0022-061-E11C-8409
GenBank Accession LN501861 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K108894-0022-061-E11C-8409
ATTTCAAAGCCTTGATTGCTTACTATTTGGTCTGTTGCATTTCAATGATTCTTTGACCAT
TAATCTTGAAACTTGGGGTTGCTAATCTCAAGATTTAACTGTAATCCATGGATTTGCACA
GACCCTACAAAGCAAACTCAGCTGGACTGGATGCAGCGATACAATATCATTGGAGGAATT
GCTCGGGGGATTCTATATCTTCATCAAGATTCACGGCTCACAATCATACACCGTGACCTT
AAAGCAAGTAACATTCTCCTAGATGCGGATATAAATCCGAAAATTGCAGATTTTGGAATG
GCTAGGATCTTCGGATTGGACCAAACCCAGGATAACACAAGCCGAATAGTTGG

Last Updated on 06.12.2018 16:11