Sequences from confirmation amplicon


Confirmation sequences for line 285A06 and hit At4g25370 are shown.
Line ID 285A06
Locus At4g25370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12974578
Primers used for PCR LG03 - 8474, show primer details
   
Sequence Name 01-K024844-022-285-A06C-8409
GenBank Accession LN486419 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K024844-022-285-A06C-8409
AGATTTCCAAAGACCCCACTTTCATTAGTATTCACTTTCCATACTTGTTTGTGGTTAGTG
TCATTTAGCTTTTCCGCATTATCACAATATACAACTCTGGCAAATGATTCAGTGCTTTGG
TTCTCCGATAGGTTTTTGTTACTAAAACTCACGTCCTCTGTTGATTATTGACACCTTGGT
GTCTTCATATGGATGCTGGGTAGGTATTTTCTTTACGCTAAAGATTGATTGTTATCTGAA
GGAATGAAACAGACGAGATAAAAAAAACTTGCAGAAGATGGAAACTGTAATTTCTTTCAA
GGGTTAAAAAGTCATTCGTCAGTCAACTGCAACGTCTAGTTGTTTCCATACTCGGTTTCA
AACAAAAGCTGACATGCTTCAGAAAACCTATCCATAGATTCAAGCTTCT
   
Sequence Name 02-K024844-022-285-A06C-LG03
GenBank Accession LN486875 [GenBank]
Sequencing primer gene specific primer, LG03: 5'-AGATGATGATGGAGAAGCTTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K024844-022-285-A06C-LG03
TTATGGAAACAACATAGACGTTGCAGTTGACTGACGAATGACTTTTTAACCCTTGAAAGA
AATTACAGTTTCCATCTTCTGCAAGTTTTTTTTATCTCGTCTGTTTCATTCCTTCAGATA
ACAATCAATCTTTAGCGTAAAGAAAATACCTACCCAGCATCCATATGAAGACACCAAGGT
GTCAATAATCAACAGAGGACGTGAGTTTTAGTAACAAAAACCTATCGGAGAACCAAAGCA
CTGAATCATTTGCCAGAGTTGTATATTGTGATAATGCGGAAAAGCTAAATGACACTAACC
ACAAACAAGTATGGAAAGTGAATACTAATGAAAGTGGGGTCTTTGGAAATCTACATGGCT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR LG02 - 8474, show primer details
   
Sequence Name 02-K028094-022-285-A06C-8409
GenBank Accession LN486896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K028094-022-285-A06C-8409
CCATTTACATTGAAATTGACCTTGTTTCTTGAAGCTCAAATCTACATCTTCATTCATCTG
CACCAAACAGACACTCAACACTTGTGACTTTGAGAAAGCTTTTCTATCCTGTAGGATTTG
GTGTTAAGAAACTTACCGATTTCTCAACTTCTTTGGCTTTATCTTCGTTAAACCCGAGCT
TCTCCAAAATCTGGCGGCCTGCTGAATCCTTTTGAGACCAAACCCCCAGAAGCAAGTAGG
CCGTGGTTAGTTCCCCATCCACATCTGTGACCAAATAGTTTCAGTTGGCAATATAATATA
ATGGATGGGAAACAGCAAGGATCCACAAAATATTAAGGAGGTGTTGTTTATGCACACCAG
ATTTATTCTTCTCATCAATGGCCCAGGCAATGGCCTTTTGAGCCGGCTCAGTTAGAGGAG
GATGCTCTGGGCTGAAAAAGTACATGTCTGATTTCCCCAGCAGGCTTAACGTCTCATCTC
GAACCTTGAAAAGTGTGACTCCATTCCCCCTCAGGAACTTAGCAACTGTGCTAGTGCCTA
AAATCAAAACCAACTATGACAAGCTTAATATACGTCAGTAAACCACATAATCCAAAACGA
TTTACCATAAATGCCTTTTGAATAACAGAAAGAAGAAGGGTATAAAACACAAAGATAAAA
CTTTCTTTCTGATGATTCCACCCAGTTCTCTCCTCTCGATCAAACCGCANAAGCATTCAA
CAACTGAATATAATAAAGTTACAGTTTTTTATGGTTACCTTATTTCACATGTATNTTGTT
TTATAGGAACAAACACAGCCGGTACTT
   
Sequence Name 02-K028094-022-285-A06C-LG02
GenBank Accession LN486897 [GenBank]
Sequencing primer gene specific primer, LG02: 5'-TCTTAACTGATTCCGAGCATACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K028094-022-285-A06C-lg2
AAATACATGTGAAATAAGGTAACCATAAAAAACTGTAACTTTATTATATTCAGTTGTTGA
ATGCTTATGCGGTTTGATCGAGAGGAGAGAACTGGGTGGAATCATCAGAAAGAAAGTTTT
ATCTTTGTGTTTTATACCCTTCTTCTTTCTGTTATTCAAAAGGCATTTATGGTAAATCGT
TTTGGATTATGTGGTTTACTGACGTATATTAAGCTTGTCATAGTTGGTTTTGATTTTAGG
CACTAGCACAGTTGCTAAGTTCCTGAGGGGGAATGGAGTCACACTTTTCAAGGTTCGAGA
TGAGACGTTAAGCCTGCTGGGGAAATCAGACATGTACTTTTTCAGCCCAGAGCATCCTCC
TCTAACTGAGCCGGCTCAAAAGGCCATTGCCTGGGCCATTGATGAGAAGAATAAATCTGG
TGTGCATAAACAACACCTCCTTAATATTTTGTGGATCCTTGCTGTTTCCCATCCATTATA
TTATATTGCCAACTGAAACTATTTGGTCACAGATGTGGATGGGGAACTAACCACGGCCTA
CTTGCTTCTGGGGGTTTGGTCTCAAAAGGATTCAGCAGGCCGCCTGATTTTGGAGAAGCT
CGGGTTTAACGAAGATAAAGCCAAAGAAGTTGAGAAATCGGTAAGTTTCTTAACACCAAA
TCCTACAGGATAGAAAAGCTTTCTCANAGTCACAAGTGTTGAGTGTCTGTTTGGTGCAGA
TGAATGAAGATGTAAATTTGAGCTTCAAGAAACAAGGTCAATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAGAGA
AAGAGTAATTACCAATTTT

Last Updated on 06.12.2018 16:11