Sequences from confirmation amplicon


Confirmation sequences for line 139F10 and hit At4g26210 are shown.
Line ID 139F10
Locus At4g26210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13282482
Primers used for PCR 12wx - 8474, show primer details
   
Sequence Name 64-K118874-0022-139-F10C-12wx
GenBank Accession LN511302 [GenBank]
Sequencing primer gene specific primer, 12wx: 5'-ACTTCCTTCCAAAATGACTCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118874-0022-139-F10C-12wx
ACAGTCAGTCATAGCAACAAGAAGACACGCAGTTTCTGAATAACGTATCTAGAAACAGTG
AAATGACTATTCTGGTAAGAAATCTACACAAATACTATACTCTATATAAGCAGGGGATTG
TTAGTATCTAGTTCAGATGCAGCAAAGTAGAGACTGTAACTAGGTATGTGATGAGTTTCA
TGGTTCAGAACCATCAAACCTAGAATGTATAATTTCACAATTCTTTTATAACACTGCCCC
TAACCAAATTTGATAACGAAGCAGGATTATCTACATCATTAGCGGGATTTCGCACAATTT
CTTGTCTAACAAACACATCAAACGAAATCCAGGATCAGATGAGAGAGCGAAGACTAACCT
AGCAAGACGAGTGTAGAGAAGCTGCTTAGACAATTCATTGCATTTCTCTATAGTAGCTGG
CTCCTGGAAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATATAGATATCC
   
Sequence Name 24-K118873-0022-139-F10C-8409
GenBank Accession LN497206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118873-0022-139-F10C-8409
ATTGATTTCCAGGAGCCAGCTACTATAGAGAAATGCAATGCATTGTCTAAGCAGCTTCTC
TACACTCGTCTTGCTAGGTTAGTCTTCGCTCTCTCATCTGATCCTGGATTTCGTTTGATG
TGTTTGTTAGACAAGAAATTGTGCGAAATCCCGCTAATGATGTAGATAATCCTGCTTCGT
TATCAAATTTGGTTAGGGGCAGTGTTATAAAAGAATTGTGAAATTATACATTCTAGGTTT
GATGGTTCTGAACCATGAAACTCATCACATACCTAGTTACAGTCTCTACTTTGCTGCATC
TGAACTAGATACTAACAATCCCCTGCTTATATAGAGTATAGTATTTGTGTAGATTTCTTA
CCAGAATAGTCATTTCACTGTTTCTAGATACGTTATTCAGAAACTGCGTGTCTTCTTTGT
TGCTATGACTGACTTGTTTTTTTCAAATGGATCGTAGCATTCCTGGGCGTTCTGAGTCAT
TTGGAAA

Last Updated on 06.12.2018 16:11