Sequences from confirmation amplicon


Confirmation sequences for line 114D03 and hit At4g26300 are shown.
Line ID 114D03
Locus At4g26300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13309063
Primers used for PCR 06fx - 8474, show primer details
   
Sequence Name 76-K111632-0022-114-D03C-06fx
GenBank Accession LN513422 [GenBank]
Sequencing primer gene specific primer, 06fx: 5'-CAATTATGATGAATGATTTGCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K111632-0022-114-D03C-06fx
ACAGCTATGTGCGGTTTTGTTAATATTCTTTCAGGTATGCTGACCTGAAGAACAACAGAT
TAACAAATTATACTTTCAGCTTTGATCAAATGCTTAATGACAAGGTACTGAAATTTGCCA
CCTAGTTTACAAATTTGCATTTTGCAGTCACCTACACAACCTTATTGATCATATGTCATG
AGCAGGGAAATACAGCCGTTTACCTTCTTTACGCCCATGCTCGGATCTGTTCAATCATCA
GAAAGTCTGGCAAAGACATAGATGAGCTGAAAAAGGTACTTTAGCTGCATGAACATGAAT
CGCTGAATTTTATTCTTTGTTCCTCTTGGCTGCCCCTGAAACTTTCCCATTGTCTCTATC
AAGGAAATGCGACGTTATAAACTATTTAGTTTGAAATTGAAAGCTTAAACAATTTTCAGA
AGTGTTTAAACTTTTAAGTTAATGCCAGTGTCTATAGTGAGCATTTAATTTTTTGGAGTC
TCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCA
   
Sequence Name 60-K111631-0022-114-D03C-8409
GenBank Accession LN510297 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K111631-0022-114-D03C-8409
ATTTACAATTGAATATATCCTGAGACTCCAAAAATTAAATGCTCACTATAGACACTGGCA
TTAACTTAAAAGTTTAAACACTTCTGAAAATTGTTTAAGCTTTCAATTTCAAACTAAATA
GTTTATAACGTCGCATTTCCTTGATAGAGACAATGGGAAAGTTTCAGGGGCAGCCAAGAG
GAACAAAGAATAAAATTCAGCGATTCATGTTCATGCAGCTAAAGTACCTTTTTCAGCTCA
TCTATGTCTTTGCCAGACTTTCTGATGATTGAACAGATCCGAGCATGGGCGTAAAGAAGG
TAAACGGCTGTATTTCCCTGCTCATGACATATGATCAATAAGGTTGTGTAGGTGACTGCA
AAATGCAAATTTGTAAACTAGGTGGCAAATTTCAGTACCTTGTCATTAAGCATTTGATCA
AAGCTGAAAGTATAATTTGTTAATCTGTTGTTCTTCAGGTCAGCATACCTTGAAAGAATA
TTAAACAAAACCGCACATTAGCTTGTAGCCATGAATATAAACCAACTGTCAAAACATCTG
GCAAATCATTCATCATAATTG

Last Updated on 06.12.2018 16:11