Sequences from confirmation amplicon


Confirmation sequences for line 839D03 and hit At4g27590 are shown.
Line ID 839D03
Locus At4g27590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13771413
Primers used for PCR 42qz - 8474, show primer details
   
Sequence Name 36-K115806-0022-839-D03C-42qz
GenBank Accession LN502354 [GenBank]
Sequencing primer gene specific primer, 42qz: 5'-TTCAAAAGGAAAGGGATCTAGTGA-3'
Sequence (A. th genome BLAST matches underlined)
>36-K115806-0022-839-D03C-42qz
TTCTATAAGTGACATAGGAGGTCTTATACTGAATCTCGGAGGCGTTGTAGAATACGTATA
AAACGCCGATGATTTATCGGTATCAAATGAAGTTTTAATAATAGTGTCTTCTTTTACGAC
AATGCAATCATCAACGCTCTCTGGATTATTATTCTCCTCTTCCTCTACATTCCAAAACCG
AGCTTCTTTTCGCTTCTTTTTCACCATCGCTAACACATCGAGTTTGTTACAAATCCCCCA
CACCGTCACCTTCTGATTGTAGTAGTCTACTTTCACTGAATATATTCCTGATTATTCGTT
ATAAAGAAAATATAATAATTATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 15-K115805-0022-839-D03C-8409
GenBank Accession LN493117 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>15-K115805-0022-839-D03C-8409
TTACATTGAATATATTCAATAATTATTATATTTTCTTTATAACGAATAATCAGGAATATA
TTCAGTGAAAGTAGACTACTACAATCAGAAGGTGACGGTGTGGGGGATTTGTAACAAACT
CGATGTGTTAGCGATGGTGAAAAAGAAGCGAAAAGAAGCTCGGTTTTGGAATGTAGAGGA
AGAGGAGAATAATAATCCAGAGAGCGTTGATGATTGCATTGTCGTAAAAGAAGACACTAT
TATTAAAACTTCATTTGATACCGATAAATCATCGGCGTTTTATACGTATTCTACAACGCC
TCCGAGATTCAGTATAAGACCTCCTATGTCACTTATTAGAACCTCTTCATTCACATGGAA
AGCCGTGAAGAAGGTCTTCACTAGATCCCTTCCCTTTTGA

Last Updated on 06.12.2018 16:11