Sequences from confirmation amplicon


Confirmation sequences for line 812F02 and hit At4g29460 are shown.
Line ID 812F02
Locus At4g29460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14482866
Primers used for PCR 65wl - 3144, show primer details
   
Sequence Name 71-K118414-0022-812-F02C-65wl
GenBank Accession LN512632 [GenBank]
Sequencing primer gene specific primer, 65wl: 5'-GCCCTTATTCGATAGTGATACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118414-0022-812-F02C-65wl
CATTTCTTCGTGGGATAGGTATTATTGAGCTCTGATTTTGTGACATTCAATATAAGCTAG
TACTAGTGATGAAACCACATGACTCTTTTTCTGTTACATTGTGACATTTCTTATGATAAG
ATATATGTGAATAATCTCTGCGCAGGTAATATATTTAATCCCCCGGTGCTGGGAAGCGTG
CCCGTCGTGGAGGTGGATCTCGCTCGGAGTAAAGTGGACACAAAGGATGGCCTTGGAACA
AAACTAGGCCTTCAAACAAAAGAAGGCTCCAAAGTCTCTGCTTCTTTAAATATATGAGCC
CTTCTAGTACTATTTCTTGATCATCTTCTAAAGTGACTACTCTCTGTAACTTCGTTGTTA
TAGAAAATTCATAAAACAAAAAAAGACTTTGAATTCTGTCCCAGATCAAAATCAACTGGC
AATCAATGACTCAGTCTATGTATTTTATCTATTATTTTGGATGTTAATCACATCTTTAAT
AACTTCACTAAAGTCAAGAAGCAGAGATACATGTTGTCTTCTAATAAAATCCAGTTGATT
AGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAAT
GAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCAT
CCCTTACGTCAGTGGAGATAT
   
Sequence Name 23-K118413-0022-812-F02C-35St
GenBank Accession LN496756 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118413-0022-812-F02C-35St
TCCACTATCCTTGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTAATCAACTGGATTTT
ATTAGAAGACAACATGTATCTCTGCTTCTTGACTTTAGTGAAGTTATTAAAGATGTGATT
AACATCCAAAATAATAGATAAAATACATAGACTGAGTCATTGATTGCCAGTTGATTTTGA
TCTGGGACAGAATTCAAAGTCTTTTTTTGTTTTATGAATTTTCTATAACAACGAAGTTAC
AGAGAGTAGTCACTTTAGAAGATGATCAAGAAATAGTACTAGAAGGGCTCATATATTTAA
AGAAGCAGAGACTTTGGAGCCTTCTTTTGTTTGAAGGCCTAGTTTTGTTCCAAGGCCATC
CTTTGTGTCCACTTTACTCCGAGCGAGATCCACCTCCACGACGGGCACGCTTCCCAGCAC
CGGGGGATTAAATATATTACCTGCGCAGAGATTATTCACATATATCTTATCATAAGAAAT
GTCACAATGTAACAGAAAAAGAGTCATGTGGTTTCATCACTAGTACTAGCTTATATTGAA
TGTCACAAAATCAGAGCTCAATAATTACCTATCCCACTGAAGAAAATGCCGTAATCCATT
CCATTGAACACGGTTGGTATCA

Last Updated on 06.12.2018 16:11