Sequences from confirmation amplicon


Confirmation sequences for line 563B03 and hit At4g29530 are shown.
Line ID 563B03
Locus At4g29530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14495942
Primers used for PCR 27ny - 8474, show primer details
   
Sequence Name 26-K114669-0022-563-B03C-27ny
GenBank Accession LN498042 [GenBank]
Sequencing primer gene specific primer, 27ny: 5'-TGTCAATCTTAAGCATCCAATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114669-0022-563-B03C-27ny
TTTCGCATCTCATGATCTCAACTCCGAGATCTACCATTTATTTCCCACCAGCAAATTCAG
TAAGCAGAAACACATCAAATCAAACTAGAAAGATCACTAAAAATACATTAAAAACTCAAC
CTTTTTGGGAGAGATCTGTTGTTGCTTCTCCTTCACTAGCTGGTGTAACCGAGAAGATAG
TTTCTCGCAAACCTGACACTTTATATACGGAACATCCTCCTTTCTCGGCGTCGAAGAAGG
TTTCTTCGCGGCATCGGAAACAGGTAATAACGCCGACGAAGCTAGCAGAGCTAATGTAAG
AGCTACCAGAACTCGATTCATCACAACCATTGAAGTCAGAAGCAGAGAAGCTTTCTCTAT
CTCTCTNAAATATGGTAAAGTTTGGGACTTTAATAAAATTCTTTCACGGGTTCTATATAG
AAGATCATGGGTTTAAAGAATTCTCTGGGTTTTGATCCTGTGCCGTGTTCATGAAGTGGA
ATCGATGTTACGCTTGTTAACGTGTTTCCTGCAATCTTATTGGCTGTTGGAATGCTGACA
CGAACTTGGGTCCACTTTCTCGACCATGTTAGATAATTACTGTTTTGTTTTTTTGTATGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT
   
Sequence Name 03-K114668-0022-563-B03C-8409
GenBank Accession LN487591 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114668-0022-563-B03C-8409
CTTTACTACAAAAAAACAAAACAGTAATTATCTAACATGGTCGAGAAAGTGGACCCAAGT
TCGTGTCAGCATTCCAACAGCCAATAAGATTGCAGGAAACACGTTAACAAGCGTAACATC
GATTCCACTTCATGAACACGGCACAGGATCAAAACCCAGAGAATTCTTTAAACCCATGAT
CTTCTATATAGAACCCGTGAAAGAATTTTATTAAAGTCCCAAACTTTACCATATTTAAGA
GAGATAGAGAAAGCTTCTCTGCTTCTGACTTCAATGGTTGTGATGAATCGAGTTCTGGTA
GCTCTTACATTAGCTCTGCTAGCTTCGTCGGCGTTATTACCTGTTTCCGATGCCGCGAAG
AAACCTTCTTCGACGCCAAGAAAGGAGGATGTTCCGTATATAAAGTGTCAGGTTTGCGAG
AAACTATCTTCTCGGTTACACCAGCTAGTGAAGGAGAAGCAACAACAGATCTCTCCCAAA
AAGGTTGAGTTTTTAATGTATTTTTAGTGATCTTTCTAGTTTGATTTGATGTGTTTCTGC
TTACTGAATTTGCTGGTGGGAAATAAATGGTAGATCTCGGAGTATGAGATCATTGAGATT
GCTGAAAATGTCTGCAATTTGAAGAAAGAGGAAGCTGATTGGA
Primers used for PCR 46vk - 8474, show primer details
   
Sequence Name 83-K117739-0022-563-B03C-46vk
GenBank Accession LN514353 [GenBank]
Sequencing primer gene specific primer, 46vk: 5'-CATCCTATCCTTTTCAAAATCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K117739-0022-563-B03C-46vk
CGTATCAAAGAAGAGGATGAGATTTTGGATGCTTTAATAAATGAAATACCATAAGACGAT
TCCATGGTAAAGTGAAACGGAGCTGATGGAAGATCTCTGTGAGTCCCATTTCAGTGACAA
CCCAATTGTCACTATCTCCGTCGATCAAAGTCCGATCGAAATCAAATAATATCACGATTT
TTGCCATTTTCTGAGATATAAACACAACGCCGGAGCTCTGCTTCTCGCCGGAGCTGCAAA
TCACTTCTCCTCTTTCTTAATTGGTCGACGGCAACATCCAATTGTCCGACGCCACTTTTC
GTCTTGCCAATGACAAGTTTACCACTCTCCATCCTATTAATTACATTTGAGCCATCCAGT
CGTATTAATTAATGGGCCTTACTGCTTGGCCCATTTTGTGCTGTGATCCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GC
   
Sequence Name 67-K117738-0022-563-B03C-8409
GenBank Accession LN511896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K117738-0022-563-B03C-8409
TCGGACATGACCATTTCATTGAATATATCCTGGATCACAGCACAAAATGGGCCAAGCAGT
AAGGCCCATTAATTAATACGACTGGATGGCTCAAATGTAATTAATAGGATGGAGAGTGGT
AAACTTGTCATTGGCAAGACGAAAAGTGGCGTCGGACAATTGGATGTTGCCGTCGACCAA
TTAAGAAAGAGGAGAAGTGATTTGCAGCTCCGGCGAGAAGCAGAGCTCCGGCGTTGTGTT
TATATCTCAGAAAATGGCAAAAATCGTGATATTATTTGATTTCGATCGGACTTTGATCGA
CGGAGATAGTGACAATTGGGTTGTCACTGAAATGGGACTCACAGAGATCTTCCATCAGCT
CCGTTTCACTTTACCATGGAATCGTCTTATGGTATTTCATTTATTAAAGCATCCAAAATC
TCATCCTCTTCTTATGATTTACGCATATTGAAATCAAAATTTGAATTGGATTTTGAAAAG
GATAGGATG

Last Updated on 06.12.2018 16:11