Sequences from confirmation amplicon


Confirmation sequences for line 306D02 and hit At4g29730 are shown.
Line ID 306D02
Locus At4g29730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14559440
Primers used for PCR 14wj - 8474, show primer details
   
Sequence Name 38-K118804-0022-306-D02C-14wj
GenBank Accession LN503161 [GenBank]
Sequencing primer gene specific primer, 14wj: 5'-AGCACTTGAGATTTGGATTTCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118804-0022-306-D02C-14wj
TGGATTCACAAAAAAAAAAGTTGAAAATACAAGCAAGTTAGGTGCCTAGGATTAACCTGA
AGAAACAGGATCAAGTACCATACAAAATTCCGGAAGTCCTAAATCTACCAATATGTTGAA
TTACTGGCCTACATAAACCAAGTAATATTCCGCAAAAATAACACCTAAATCGCATAAGTA
AACTGATTTTCCTCGCAAAAACTGACACTAAACCCCAGCAACTTACTAATACTAATAGAC
ATGATGGGATTGCTTCCTCAAAACTTGGAGGTACATGTGAAAACATGTGACTTGAATTTC
TCTAGCTCTGTCAGGACTTCATCTTCCGGTCTGTAAATCAAGTCACTCATCCGCCATATC
TGCATTAATTTACAAATCAAAAGTTTTCATTTTTACCGAGAAAGTTAATCATAGGTAGGT
TCCAAAGTAAATGTAGCAAGACAGAGTATACCGGTTTCTCCATATTTACATTGTTTATTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTC
C
   
Sequence Name 19-K118803-0022-306-D02C-8409
GenBank Accession LN494976 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118803-0022-306-D02C-8409
TTGAAATAAACAATGTAAATATGGAGAAACCGGTATACTCTGTCTTGCTACATTTACTCT
GGAACCTACCTATGATTAACTTTCTCGGTAAAAATGAAAACTTTTGATTTGTAAATTAAT
GCAGATATGGCGGATGAGTGACTTGATTTACAGACCGGAAGATGAAGTCCTGACAGAGCT
AGAGAAATTCAAGTCACATGTTTTCACATGTACCTCCAAGTCTTGAGGAAGCAATCCCAT
CATGTCTATTAGTATTAGTAAGTTGCTGGGGTTTAGTGTCAGTTTTTGCGAGGAAAATCA
GTTTACTTATGCGATTTAGGTGTTATTTTTGCGGAATATTACTTGGTTTATGTAGGCCAG
TAATTCAACATATTGGTAGATTTAGGACTTCCGGAATCTTGTATGGTACTTGATCCTGTT
TCTTCAGGTTAATCCTAGGCACCTAACTTGCTTGTATTTTCAACTTTTTTTTTTGTGAAT
CCAAACTTGGTTAAGCAAGACCAAACTAAAACAACAGATTGAATCAGTGAAATCCAAA

Last Updated on 06.12.2018 16:11