Sequences from confirmation amplicon


Confirmation sequences for line 793C03 and hit At4g29735 are shown.
Line ID 793C03
Locus At4g29735 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14564402
Primers used for PCR BM41 - 8474, show primer details
   
Sequence Name 34-K104374-0022-793-C03C-BM41
GenBank Accession LN501403 [GenBank]
Sequencing primer gene specific primer, BM41: 5'-CAATATATGTGGTCGCAAATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104374-0022-793-C03C-BM41
TCGGCAGCCGGTTTTCGTCATTTAGTATGGCAACACTTCTTTGGGACCATATTTTCGTAT
TGGTCAACAAATTAAACCCTAACCAATTTCTAAGTCCTTTCCATGAAAAAAACGAAATCT
ATAAGTCATTGTTGACCAATACAAAAATCTGTTACTATTACATCCGGTCCCATATTTTCA
GGTGGGAACTAAATCTAGAGAGAGTACCCAATCTTCAATCCGGAAATTGATTGACAAGGA
GTTGGAGATGTCTACATTGAAGTGGATTTCAATTTTCGAAAATGGTAAAGGATCACATTG
CACTTTGAATAAAGTTATACAACGTTAAAAGAAGATAAAGATATGAACACATTTTATATC
AAAGGCCTTAATAAGTATATTTGGGCCTATTAAATCGAACCTTTAAACAGAAAAACTGGC
CCATAAAATAACCGTGGTTGTGTTATAACAAGAAAGAAAGAGTAAATCTACATGGCTAAA
TTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCG
   
Sequence Name 50-K104375-0022-793-C03C-8409
GenBank Accession LN507158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K104375-0022-793-C03C-8409
TTTACAATTAAATTTAGCCATGTAGATTTACTCTTTCTTTCTTGTTATAACACAACCACG
GNTTATTTTATGGGCCAGTTTTTCTGTTTAAAGGTTCGATTTAATAGGCCCAAATATACT
TATTAAGGCCTTTGATATAAAATGTGTTCATATCTTTATCTTCTTTTAACGTTGTATAAC
TTTATTCAAAGTGCAATGTGATCCTTTACCATTTTCGAAAATTGAAATCCACTTCAATGT
AGACATCTCCAACTCCTTGTCAATCAATTTCCGGATTGAAGATTGGGTACTCTCTCTAGA
TTTAGTTCCCACCTGAAAATATGGGACCGGATGTAATAGTAACAGATTTTTGTATTGGTC
AACAATGACTTATAGATTTCGTTTTTTTCATGGAAAGGACTTAGAAATTGGTTAGGGTTT
AATTTGTTGACCAATACGAAAATATGGTCCCAAAGAAGTGTTGCCATACTAAATGACGAA
AACCGGCTGCCGAATATTTCTATAGGTAGGACATAGAACTTACATCAATTTGCG

Last Updated on 06.12.2018 16:11