Sequences from confirmation amplicon


Confirmation sequences for line 111D01 and hit At4g30190 are shown.
Line ID 111D01
Locus At4g30190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14770835
Primers used for PCR 14pm - 8474, show primer details
   
Sequence Name 39-K115064-0022-111-D01C-14pm
GenBank Accession LN503459 [GenBank]
Sequencing primer gene specific primer, 14pm: 5'-AGCCGTACTACTTCCACACAACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115064-0022-111-D01C-14pm
CATTTTTAAGGATAGTGTTATTAATTGATTTGGTTTCGTGAAAAAAAAAAAAAAAAAATT
ATGGTTATTTCCCTTCTTTACAACAAACAAAATTTGATTTAAATAACCAAATTGTTTGTT
GTCCCTAGGTATAAATAACATTTAGGTACTATATATAAAATTAAAAAACCCGGAAAAAAA
TTTGGATTACAAAAAAAAAAAAAATTAAAATTACTTAGGCTCAGGGAATAAAAGCAAAAA
AAAAAATGTGCATGTGCCAAATGCCAAACACAAAGGCATGCCTTGTTTCTTCAAAAAAGC
AAATTATGCAAAATTACATATTTGAAAAACAAAAACACTAACAATAAGGTTTGGGAAAAT
TTATGGCAATAAACAAAAATCTCTCCCTATGTAAATAAAAATCAAAAAAGGTGGTGAAGA
AAAAAAGAAAAAAAAAAAAACAAACCTTGAGAGGATCGAAAATCATTCATATTTTTGGTT
TTACAACACAGTGTAGTGATCAGTCAAATTTTTGCTCATTGAAAATATCCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACTTGGATAACCATTGAGTATAATGGTCAATATGGAAAA
AAAAAAAAAGTATTTACCATTTTTTTTTCATTTCAAAAATGTAAATTTCC
   
Sequence Name 07-K115063-0022-111-D01C-8409
GenBank Accession LN489397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115063-0022-111-D01C-8409
CATTTCATTGAATATATCCATGAGCAACATATGACTGATCACTACACTGTGTAGTAAAAC
CAAACATATGACTGATCTTCGATCCTCTCATGTTTTGTCTTCTTCTTCTTCTTTTTTTCT
TCACCACCTCTTATGATTATTATTCACATAGGGAGAGATCTTTGTTTATTGCCATAACTC
TTCCCAAACCTTATTGTTAGTGTTCTTGTTTCTCAAATATGTACTTTTGCATAATTTGCT
CTTTTGAAGAAACAAGGCATGCCTTTGTGTTTGGCATTTGGCACATGCACATTCTCTTCT
TTGCTCTTATTCCATGAGCCTATGTAATCTTAATCTTCTTCTTTTTCGTAATCCAACTTT
TCTTCCGGCTTCTTTACTTTTATATATAGTACCTAAATGTTATTTATACCTAGGGACAAC
AAACAATTTGCTTATTTAAATCAAATTTTGTTTGTTGTAAAGAAGGGAAATAACCATAAT
TTTTTCTTTTTTTTTTTCACTGAAACCAAATCATTTAAATAACACTATCCTTTAAAAATG
CCTTTAAAAACATGACAGGAAACAATTTGTGTGAAA

Last Updated on 06.12.2018 16:11