Sequences from confirmation amplicon


Confirmation sequences for line 219D04 and hit At4g30190 are shown.
Line ID 219D04
Locus At4g30190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14773082
Primers used for PCR MJ14 - 8474, show primer details
   
Sequence Name 26-K021339-022-219-D04C-8409
GenBank Accession LN497729 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K021339-022-219-D04C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAAATGTTCAACTGTTTGTCTTCTT
ATTCCCTTTTGTAGGTGGTGCCAGAGAAAACAAAGGAAAGCCCAGGTGCGCCATGGGAAT
TTGTTGGCTTGTTGCCACTTTTTGATCCCCCAAGACATGACAGTGCTGAAACAATTCGAC
GGGCTTTGAATCTTGGTGTTAACGTCAAGATGATCACTGGTAAATTTGTCTACCCATCTA
TCTTGTTGTGCTAGCTATACACCTTCACAAGTTCCCCCTAACATTCTCTTGAACACATAT
GTGGCCAGGTGACCAACTTGCTATTGGTAAGGAAACTGGTCGCAGACTTGGAATGGGAAC
AAACATGTATCCATCTTCGGCTCTTCTTGGTACACACAAAGACGCAAACCTCGCATCCAT
TCCTGTTGAGGAGTTGATTGAAAAGGCTGATGGATTTGCCGGAGTCTTCCCAGGTTATAA
TCTGCTTATTTATTGTTTGGATTATAAACCTCACTATATGTTCATTGCAAAGGTGGTGAT
GTTAGTTCTAAGCTTTGTTTTTTTTATTGCAGAGCACAAATACGAAATTGTGAAAAAGTT
GCAGGAGAGGAAGCATATTGTTG
   
Sequence Name 27-K021339-022-219-D04C-MJ14
GenBank Accession LN498191 [GenBank]
Sequencing primer gene specific primer, MJ14: 5'-ATCACCAGTCATTCCAACAATATG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K021339-022-219-D04C-MJ14
CTCTCCTGCAACTTTTTCACAATTTCGTATTTGTGCTCTGCAATAAAAAAAACAAAGCTT
AGAACTAACATCACCACCTTTGCAATGAACATATAGTGAGGTTTATAATCCAAACAATAA
ATAAGCAGATTATAACCTGGGAAGACTCCGGCAAATCCATCAGCCTTTTCAATCAACTCC
TCAACAGGAATGGATGCGAGGTTTGCGTCTTTGTGTGTACCAAGAAGAGCCGAAGATGGA
TACATGTTTGTTCCCATTCCAAGTCTGCGACCAGTTTCCTTACCAATAGCAAGTTGGTCA
CCTGGCCACATATGTGTTCAAGAGAATGTTAGGGGGAACTTGTGAAGGTGTATAGCTAGC
ACAACAAGATAGATGGGTAGACAAATTTACCAGTGATCATCTTGACGTTAACACCAAGAT
TCAAAGCCCGTCGAATTGTTTCAGCACTGTCATGTCTTGGGGGATCAAAAAGTGGCTACA
AGCCAACAAATTCCCATGGCGCACCTGGGCTTTCCTTTGTTTTCTCTGGCACCACCTACA
AAAGGGAATAAGAAGACAAACAGTTGAACATTTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTAT

Last Updated on 06.12.2018 16:11